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Corallincola luteus sp. nov., a marine bacterium isolated from surface sediment of Bohai Sea of China

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Abstract

A novel Gram-stain negative, strictly aerobic, rod-shaped motile bacterium with a single flagellum, designated strain DASS28T, was isolated from surface sediment of Bohai Sea in China. Growth occurred in the presence of 1.0–4.0% NaCl (w/v, optimum 2.0%), at 10–37 °C (optimum 20 °C) on the Marine agar 2216E and pH 6.0–10.0 (optimum pH 8.0). The major fatty acids (> 10% of total fatty acids) were summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH), C16:0 and C18:1ω7c. The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, an unidentified aminolipid and two unidentified polar lipids. The major respiratory quinone was ubiquinone-8 (Q-8). The genomic DNA G + C content calculated from the genome sequence of strain DASS28T was 48.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain DASS28T belongs to the genus Corallincola and shows high 16S rRNA gene sequence similarity of 96.7% to Corallincola platygyrae JLT 2006T (= JCM18796T = CGMCC 1.10992T). On the basis of the polyphasic evidence, strain DASS28T is considered to represent a novel species in the genus Corallincola, for which the name Corallincola luteus sp. nov. is proposed. The type strain is DASS28T (= KCTC 52376T = MCCC 1K03208T).

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Abbreviations

HPLC:

High performance liquid chromatography

JCM:

Japan Collection of Microorganisms

MK-7:

Menaquinone 7

Q-8:

Ubiquinone-8

PG:

Phosphatidylglycerol

PE:

Phosphatidylethanolamine

PL:

Phospholipid

AL:

Aminolipid

UL:

Unidentified polar lipid

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Acknowledgements

We are grateful to the captain and crew of the R/V Dong Fang Hong 2 for their assistance during the cruise and the kind help of Prof. Aharon Oren for the naming of the novel strain DASS28T. This work was supported by projects from the National Key Research and Development Program of China (No. 2016YFA0601303) and the National Natural Science Foundation of China (Nos. 41476112 and 41521064).

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Author notes

  1. Qiaomeng Ren and Xiaoting Yang have contributed equally to this work.

Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China

    Qiaomeng Ren, Xiaoting Yang, Min Yu, Xiaorong Tian, Bei Li, Zhe Meng, Yanhong Wu, Jiwen Liu & Xiao-Hua Zhang

  2. Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China

    Min Yu & Xiao-Hua Zhang

  3. Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, 266003, China

    Min Yu & Xiao-Hua Zhang

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  1. Qiaomeng Ren
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  3. Min Yu
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Contributions

Qiaomeng Ren and Xiaoting Yang carried out the laboratory work, participated in the data analyzation and drafted the manuscript. Min Yu, Xiaorong Tian and Bei Li participated in the design of the study and helped to draft the manuscript. Zhe Meng, Yanhong Wu and Jiwen Liu participated in the data analyzation. Xiao-Hua Zhang conceived the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.

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Correspondence to Min Yu.

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The GenBank accession number for the 16S rRNA gene sequence of Corallincola luteus DASS28T is KU315484.1. The Whole Genome Shotgun project of strain DASS28T has been deposited at DDBJ/ENA/GenBank under the accession SJXE00000000. The version described in this paper is version SJXE01000000.

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Ren, Q., Yang, X., Yu, M. et al. Corallincola luteus sp. nov., a marine bacterium isolated from surface sediment of Bohai Sea of China. Antonie van Leeuwenhoek 112, 1691–1697 (2019). https://doi.org/10.1007/s10482-019-01295-3

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