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Algoriphagus litoralis sp. nov., isolated from the junction between the ocean and a freshwater lake

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Abstract

A Gram-stain negative, non-motile and short rod shaped bacterium, designated strain DSL-12T, was isolated from seawater of the East China Sea and characterised phylogenetically and phenotypically. Optimal growth was found to occur at 28 °C (range 4–40 °C), pH 7 (range 6–12) and with 3% (w/v) NaCl (range 0–8%). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain DSL-12T is related to members of the genus Algoriphagus and shares high sequence similarities with Algoriphagus boritolerans DSM 17298T (97.6%) and Algoriphagus alkaliphilus DSM 22703T (97.6%). The 16S rRNA gene sequence identities between strain DSL-12T and other current members of the genus Algoriphagus were below 96.4%. The digital DNA–DNA hybridization values between strain DSL-12T and the type strains A. boritolerans DSM 17298T and A. alkaliphilus DSM 22703T were found to be 21.2 ± 2.4% and 20.2 ± 2.4%, respectively. The average nucleotide identity (ANI) values between strain DSL-12T and A. boritolerans DSM 17298T and A. alkaliphilus DSM 22703T were found to be 83.2% and 82.8%, respectively. The sole respiratory quinone was identified as MK-7. The major polar lipids were identified as phosphatidylethanolamine, diphosphatidylglycerol, one unidentified phospholipid and two unidentified lipids. The major fatty acids identified as were iso-C15:0, summed feature 8 (C18:1ω7c and/or C18:1ω6c), C16:0, summed feature 3 (C16:1ω7c and/or C16:1ω6c) and iso-C17:0. The G+C content of the genomic DNA was determined to be 43.3 mol%. The combined genotypic and phenotypic data indicate that strain DSL-12T represents a novel species of the genus Algoriphagus, for which the name Algoriphagus litoralis sp. nov. is proposed, with the type strain DSL-12T (= KCTC 62647T = MCCC 1K03536T).

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Abbreviations

ANI:

Average nucleotide identity

DDH:

DNA–DNA hybridization

DPG:

Diphosphatidylglycerol

PE:

Phosphatidylethanolamine

PL:

Phospholipid

PC:

Phosphatidylcholine

AL:

Aminolipid

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Acknowledgements

This work is supported support by the National Natural Science Foundation of China (41773069, 91328208 and 41373071 to JF). YLW acknowledges the Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars State Education Ministry (Grant No. D-8002-15-8004).

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Authors and Affiliations

  1. Shanghai Engineering Research Center of Hadal Science and Technology, College of Marine Sciences, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China

    Yuli Wei, Haiyan Mao, Ke Wang, Yan Zhang, Junwei Cao, Zhe Xie & Jiasong Fang

  2. State Key Laboratory of Geological Processes and Mineral Resources, Faculty of Earth Sciences, China University of Geosciences, Wuhan, 430074, People’s Republic of China

    Huimin Yao

  3. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, People’s Republic of China

    Jiasong Fang

  4. Department of Natural Sciences, Hawaii Pacific University, Honolulu, HI, 96813, USA

    Jiasong Fang

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  1. Yuli Wei
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  2. Haiyan Mao
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  3. Ke Wang
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  4. Huimin Yao
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  5. Yan Zhang
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Correspondence to Yuli Wei or Jiasong Fang.

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Wei, Y., Mao, H., Wang, K. et al. Algoriphagus litoralis sp. nov., isolated from the junction between the ocean and a freshwater lake. Antonie van Leeuwenhoek 112, 1545–1552 (2019). https://doi.org/10.1007/s10482-019-01280-w

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  • DOI: https://doi.org/10.1007/s10482-019-01280-w

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