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Effect of nicotine from tobacco root exudates on chemotaxis, growth, biocontrol efficiency, and colonization by Pseudomonas aeruginosa NXHG29

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Abstract

Accumulated evidence suggests that root exudates have a major role in mediating plant–microbe interactions in the rhizosphere. Here, we characterized tobacco root exudates (TREs) by GC–MS and nicotine, scopoletin, and octadecane were identified as three main components of TREs. Qualitative and quantitative chemotaxis assays revealed that Pseudomonas aeruginosa NXHG29 with antagonistic activity displayed positive chemotactic responses towards TREs and their three main components (nicotine, scopoletin, octadecane) and its enhanced chemotaxis were induced by these substances in a concentration-dependent manner. Furthermore, following GC–MS and chemotaxis analysis, nicotine was selected as the target for evaluation of the effect on NXHG29 regarding antagonism, growth, root colonization and biocontrol efficiency. Results of in vitro studies showed that nicotine as a sole carbon source could enhance growth of NXHG29 and significantly increased the antagonism of NXHG29. We also demonstrated that nicotine exerted enhancing effects on the colonization ability of NXHG29 on tobacco roots by combining CLSM observations with investigation of population level dynamics by selective dilution plating method. Results from greenhouse experiments suggested nicotine exhibited stimulatory effects on the biocontrol efficiency of NXHG29 against bacterial wilt and black shank on tobacco. The stimulatory effect of nicotine was affected by the concentration and timing of nicotine application and further supported by the results of population level of NXHG29 on tobacco roots. This is the first report on the enhancement effect of nicotine from TREs on an antagonistic bacterium for its root colonization, control of soil-borne pathogens, regarding the chemotaxis and in vitro antagonism and growth.

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Acknowledgements

This research was financially supported by the National Natural Science Foundation Program of China (31200086, 31660023, 31460028, 31570108), Department of Science and Technology of Yunnan Province (2017FB020, 2017FA016), and China Tobacco Company (110201802010).

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  1. Li Ma and Shuai Chao Zheng have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, No.2 Cuihubei road, Kunming, 650091, People’s Republic of China

    Li Ma, Shuai Chao Zheng, Xing Kui Zhou, Cheng Gang Yang, Jin Ling Duo & Ming He Mo

  2. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Li Ma, Shuai Chao Zheng & Ming He Mo

  3. Pu’er Branch of Yunnan Tobacco Company, Pu’er, 665000, People’s Republic of China

    Ti Kun Zhang, Zi Yi Liu & Xue Jian Wang

  4. Yunnan Corporation of China National Tobacco Corporation, Kunming, 650202, People’s Republic of China

    Ti Kun Zhang, Zi Yi Liu & Xue Jian Wang

  5. Biocontrol Engineering Research Center of Plant Disease & Pest, Yunnan University, Kunming, 650091, People’s Republic of China

    Xing Kui Zhou & Cheng Gang Yang

  6. Biocontrol Engineering Research Center of Crop Disease & Pest, Yunnan University, Kunming, 650091, Yunnan Province, People’s Republic of China

    Li Ma, Shuai Chao Zheng, Ti Kun Zhang, Zi Yi Liu, Xue Jian Wang, Xing Kui Zhou, Cheng Gang Yang, Jin Ling Duo & Ming He Mo

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  1. Li Ma
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Ma, L., Zheng, S.C., Zhang, T.K. et al. Effect of nicotine from tobacco root exudates on chemotaxis, growth, biocontrol efficiency, and colonization by Pseudomonas aeruginosa NXHG29. Antonie van Leeuwenhoek 111, 1237–1257 (2018). https://doi.org/10.1007/s10482-018-1035-7

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