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Isolation and characterisation of an enterocin P-producing Enterococcus lactis strain from a fresh shrimp (Penaeus vannamei)

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Abstract

Screening for lactic acid bacteria (LAB) from fresh shrimp samples (Penaeus vannamei) collected from retail seafood markets in the Tunisian’s coast, resulted in the isolation of an Enterococcus strain termed Q1. This strain was selected for its antagonistic activity against pathogenic bacteria such as Listeria monocytogenes, Pseudomonas aeruginosa, Lactococcus garvieae and against fungi (Aspergillus niger and Fusarium equiseti). The Q1 strain was characterised using standard morphological and biochemical tests, growth assays at different temperatures, pH and salinity. 16S rRNA, rpoA and pheS gene sequencing, as well as the 16S-23S rRNA intergenic spacer analyses, were combined to identify strain Q1 as a strain of Enterococcus lactis. The bacteriocin produced by E. lactis Q1 is thermostable, active in the pH range from 4.0 to 9.0 and has a bactericidal mode of action. The enterocin P structural gene was detected by specific PCR in strain E. lactis Q1, which is in good agreement with SDS-PAGE data of the purified bacteriocin. A lack of significant antibiotic resistance genes and virulence determinants was confirmed by specific PCRs. This work provides the first description of an enterocin P producer E. lactis strain isolated from a fresh shrimp. Based on its safety properties (absence of haemolytic activity, virulence factors and antibiotic resistance genes), this strain has the potential to be used as a natural additive or adjunct protective culture in food biopreservation and/or probiotic culture.

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Acknowledgements

This work was supported by a Grant from the Ministry of High Education, Tunisia.

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Authors and Affiliations

  1. Laboratory of Biochemistry and Technobiology LR01ES05, Faculty of Science of Tunis, University of Tunis El-Manar, Campus Universitaire El-Manar II Tunis, Tunis, Tunisia

    Olfa Ben Braïek, Hamdi Ghomrassi, Omrane Bel Hadj & Taoufik Ghrairi

  2. Institute of Agricultural Biology and Biotechnology, Italian National Research Council (CNR IBBA), Via Einstein s/n, 26900, Lodi, Italy

    Paola Cremonesi

  3. Institute of Sciences of Food Production, Italian National Research Council (CNR ISPA), Via Celoria 2, 20133, Milan, Italy

    Stefano Morandi

  4. Université de Brest, EA3882, Laboratoire Universitaire de Biodiversité et d’Ecologie Microbienne, IUT de Quimper, 6 Rue de l’Université Quimper, 29000, Cedex, France

    Yannick Fleury & Patrick Le Chevalier

  5. Department of Biochemistry, Faculty of Medicine, Ibn El Jazzar, UR12-ES03, Sousse, Tunisia

    Khaled Hani

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  1. Olfa Ben Braïek
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Ben Braïek, O., Ghomrassi, H., Cremonesi, P. et al. Isolation and characterisation of an enterocin P-producing Enterococcus lactis strain from a fresh shrimp (Penaeus vannamei). Antonie van Leeuwenhoek 110, 771–786 (2017). https://doi.org/10.1007/s10482-017-0847-1

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