Abstract
During an investigation of microbial diversity in medicinal herbs, a novel actinobacterium, strain NEAU-KD1T was isolated from the rhizosphere of Peucedanum praeruptorum Dunn collected from Xianglu Mountain in Heilongjiang Province, northeast China and characterized using a polyphasic approach. The organism was found to have the typical chemotaxonomic and morphological characteristics of the genus Mumia. Cells were observed to be non-spore-forming and irregular cocci. The cell wall was found to contain LL-diaminopimelic acid as the cell wall diamino acid. The whole-cell sugars were detected as galactose and rhamnose and the predominant menaquinone was identified as MK-9(H4). The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid and five unidentified phospholipids. The major cellular fatty acids were determined to be composed of C16:0, 10-methyl C18:0 and C18:1ω7c. The phylogenetic analysis based on 16S rRNA gene sequence also indicated that strain NEAU-KD1T belongs to the genus Mumia and with high sequence similarity to Mumia flava NBRC 109973T (97.6 % sequence similarity). The results of DNA–DNA hybridization and the phenotypic characteristics indicated that strain NEAU-KD1T could be distinguished from its close phylogenetic relative. Thus, strain NEAU-KD1T can be concluded to represent a novel species of the genus Mumia, for which the name Mumia xiangluensis sp. nov. is proposed. The type strain is NEAU-KD1T (=CGMCC 4.7305T = DSM 101040T).
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This work was supported in part by grants from the National Outstanding Youth Foundation (No. 31225024), the National Natural Science Foundation of China (Nos. 31471832, 31171913, 31500010, 31572070 and 31372006), the National Key Technology R&D Program (No. 2012BAD19B06) and Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP).
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Zhou, S., Jia, F., Liu, C. et al. Mumia xiangluensis sp. nov., isolated from the rhizosphere of Peucedanum praeruptorum Dunn. Antonie van Leeuwenhoek 109, 827–832 (2016). https://doi.org/10.1007/s10482-016-0682-9
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DOI: https://doi.org/10.1007/s10482-016-0682-9