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Paenibacillus gelatinilyticus sp. nov. a psychrotolerant bacterium isolated from a reclaimed soil and amended description of Paenibacillus shenyangensis

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Abstract

A rod shaped, Gram-stain positive, non-motile, facultative anaerobic and gelatin hydrolysing bacterium, strain PG1T, was isolated from reclaimed land soil in Kyehwa-do, Republic of Korea. Strain PG1T showed highest 16S rRNA gene sequence similarity (97.4 and 96.5 %, respectively) to Paenibacillus shenyangensis A9T and Paenibacillus hunanensis FeL05T, and clustered closely with the members of the family Paenibacillaceae. DNA–DNA hybridization studies revealed a genomic relatedness of 47 ± 9 % with P. shenyangensis A9T. The predominant fatty acids of strain PG1T were identified to be anteiso-C15:0 (46.7 %) and C16:0 (22.7 %). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid were found to be the major polar lipids. The genomic DNA G+C content was found to be 47.7 mol%. This polyphasic characterisation of the newly isolated strain PG1T justifies its description as representative of a novel species in the genus Paenibacillus, for which the name Paenibacillus gelatinilyticus sp. nov., (type strain = PG1T = KCTC 33642T = JCM 30624T) is proposed.

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Acknowledgments

We thank Prof. Aharon Oren for his expert suggestion for correct species epithet and Latin etymology. This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ009801)” Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Division of Biotechnology, Chonbuk National University, Iksan, 570-752, Republic of Korea

    Shalem Raj Padakandla, Gun-Woong Lee & Jong-Chan Chae

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  1. Shalem Raj Padakandla
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  2. Gun-Woong Lee
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  3. Jong-Chan Chae
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Correspondence to Jong-Chan Chae.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain PG1T is KP231650.

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Padakandla, S.R., Lee, GW. & Chae, JC. Paenibacillus gelatinilyticus sp. nov. a psychrotolerant bacterium isolated from a reclaimed soil and amended description of Paenibacillus shenyangensis . Antonie van Leeuwenhoek 108, 1197–1203 (2015). https://doi.org/10.1007/s10482-015-0574-4

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