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Paenibacillus wenxiniae sp. nov., a nifH gene -harbouring endophytic bacterium isolated from maize

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Abstract

A novel Gram-positive, aerobic, motile, endospore-forming, rod-shaped bacterium, designated 373T was isolated from surface-sterilised root tissue of a maize planted in Fangshan District of Beijing, Peopole’s Republic of China. A polyphasic taxonomic study was performed on the new isolate. On the basis of 16S rRNA gene sequence similarity studies, this isolate belongs to the genus Paenibacillus. The highest 16S rRNA gene sequence similarity was found between strain 373T and Paenibacillus hunanensis (98.1 %), meanwhile the 16S rRNA gene sequence similarity between strain 373T and the type strains of other recognised members of the genus Paenibacillus were all below 95.6 %. However, the DNA–DNA hybridization values between strain 373T and the type strain P. hunanensis DSM 22170T was 30.2 %. The DNA G+C content of strain 373T was determined to be 46.0 mol%. The predominant respiratory quinone was identified as menaquinone-7 and the polar lipid profile was found to be composed of the major lipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were found to consist of anteiso-C15: 0 (59.6 %), anteiso-C17: 0 (12.8 %) and C16: 0 (6.7 %). The results of physiological and biochemical tests and minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain 373T from the closely related species in this genus Paenibacillus. Strain 373T is concluded to represent a novel species within the genus Paenibacillus, for which the name Paenibacillus wenxiniae sp. nov. is proposed, with the type strain 373T (= CGMCC 1.15007 T = DSM100576 ).

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Acknowledgments

This work was supported by “Special Fund for Agro-scientific Research in the Public Interest” (201203045) and National Natural Science Foundation of China (No. 31570008).

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    Authors and Affiliations

    1. Beijing Agro- Biotechnology Research Center, Beijing Academy of Agriculture and Forestry/Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing, 100097, People’s Republic of China

      Jun-lian Gao, Xu-ming Wang, Tian-lei Qiu, Ji-wei Li & Yi Zhou

    2. Key Laboratory of Microbial Resources, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

      Fan-yang Lv, Mei Yuan & Jian-guang Sun

    3. College of Agriculture, Yangtze University, Jingzhou, Hubei, 434025, People’s Republic of China

      Ji-wei Li & Yi Zhou

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    1. Jun-lian Gao
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    Correspondence to Jian-guang Sun.

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    Jun-lian Gao and Fan-yang Lv have contributed equally to this work.

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    Gao, Jl., Lv, Fy., Wang, Xm. et al. Paenibacillus wenxiniae sp. nov., a nifH gene -harbouring endophytic bacterium isolated from maize. Antonie van Leeuwenhoek 108, 1015–1022 (2015). https://doi.org/10.1007/s10482-015-0554-8

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