Abstract
Two novel actinobacteria, strains NEAU-CX1T and NEAU-JC6T, were isolated from nymphs of stinkbug (Palomena viridissima Poda) and a beetle (Harpalus sinicus Hope), respectively, collected from Harbin, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The phylogenetic analysis based on 16S rRNA gene sequence of strain NEAU-CX1T showed it to be most closely related to Micromonospora coxensis JCM 13248T (99.3 % sequence similarity), Micromonospora purpureochromogenes DSM 43821T (99.1 %) and Micromonospora halophytica JCM 3125T (98.6 %), and that of strain NEAU-JC6T to Micromonospora haikouensis DSM 45626T (99.3 %), Micromonospora carbonacea JCM 3139T (99.1 %) and Micromonospora krabiensis JCM 12869T (99.1 %). The phylogenetic analysis based on gyrB gene sequence of strain NEAU-CX1T showed it to be most closely related to M. purpureochromogenes DSM 43821T (98.0 % sequence similarity), and that of strain NEAU-JC6T to M. haikouensis DSM 45626T (99.0 %) and M. carbonacea JCM 3139T (98.0 %). A combination of DNA–DNA hybridization results and cultural and physiological properties indicated that the two strains can be distinguished from their closest phylogenetic relatives. Thus, strains NEAU-CX1T and NEAU-JC6T represent two novel species of the genus Micromonospora, for which the names Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. are proposed. The type strains are NEAU-CX1T (=CGMCC 4.7175T = JCM 30056T) and NEAU-JC6T (=CGMCC 4.7173T = JCM 30055T).
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Acknowledgments
We thank Dr. Bernhard Schink for his valuable help with naming the species. This work was supported in part by grants from the National Outstanding Youth Foundation (No. 31225024), the National Key Project for Basic Research (No. 2010CB126102), the National Key Technology R&D Program (No. 2012BAD19B06), the National Natural Science Foundation of China (No. 31372006, 31171913, 31071750 and 31471832), the Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP), the Science and Technology Research Project of Heilongjiang Provincial Educational Commission (No. 12541001) and the Youth Science Foundation of Heilongjiang Province (No. QC2014C013).
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Fang, B., Liu, C., Guan, X. et al. Two new species of the genus Micromonospora: Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. isolated from the insects. Antonie van Leeuwenhoek 108, 141–150 (2015). https://doi.org/10.1007/s10482-015-0472-9
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DOI: https://doi.org/10.1007/s10482-015-0472-9