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Nioella nitratireducens gen. nov., sp. nov., a novel member of the family Rhodobacteraceae isolated from Azorean Island

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Abstract

A novel Gram-negative, non-spore forming, rod-shaped aerobic bacterium, designated SSW136T, was isolated from a surface seawater sample collected at Espalamaca (in Faial Island), Azores. Growth was found to occur from 10 to 37 °C, pH 6.0–8.0, and with 2–11 % of NaCl. 16S rRNA gene sequence indicated that the strain SSW136T belongs to the family Rhodobacteraceae. Strain SSW136T exhibited 96.3, 95.9, 95.7 and 95.5 sequence similarity to the type strains Oceanicola litoreus M-M22T, Roseovarius aestuarii SMK-122T, Marivita geojedonensis DPG-138T, and Pseudoruegeria aquimaris SW-255T respectively. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSW136T was affiliated to the family Rhodobacteraceae and formed a separate branch. The G+C content was 63.5 mol%. The major respiratory quinone was found to be Q-10. The polar lipids of strain SSW136T consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and three unidentified phospholipids. The major fatty acids were C18:1 ω7c (46.5 %), Cyclo-C19:0 ω8c (16.0 %) and C16:0 (12.8 %). On the basis of the morphological, genotypic, chemotaxonomic characteristics and low DNA–DNA relatedness, strain SSW136T is proposed to represent a novel genus and novel species, Nioella nitratireducens gen. nov., sp. nov., in the family Rhodobacteraceae. The type strain is SSW136T (=KCTC 32417T = NCIM 5499T).

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Acknowledgments

The authors are grateful to Dr. N. Ramaiah and R. M. Meena for providing DNA sequence facility. Author Rajasabapathy acknowledges CSIR for providing senior research fellowship. We acknowledge Department of Science & Technology, Govt. of India for the support through an Indo-Portugal bilateral program. We acknowledge MMRF-COMAPS under ICMAM, MoES for FAME facility supported by Dr. Shanta Nair, Dr. Anas Abdulaziz and V. Vijitha. We thank Dr. V. K. Banakar and Mr. Sarath for SEM analysis. IMAR-DOP/UAz is Research and Development Unit #531 and LARSyS—Associated Laboratory # 9 funded by the Portuguese Foundation for Science and Technology (FCT) through PEst project (Pest/OE/EEI/LA0009/2011–2014), and by DRCTC—Regional Government of the Azores through a Pluriannual Funding scheme. This is CSIR-NIO contribution number 5686.

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Authors and Affiliations

  1. Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, 403 004, Goa, India

    Raju Rajasabapathy & Chellandi Mohandass

  2. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Jung-Hoon Yoon

  3. CSIR-National Chemical Laboratory, NCIM Resource Center, Dr. Homi Bhabha road, Pune, 411 008, India

    Syed Gulam Dastager

  4. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People’s Republic of China

    Qing Liu, Thi-Nhan Khieu & Wen-Jun Li

  5. School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi, Vietnam

    Thi-Nhan Khieu & Chu Ky Son

  6. IMAR-Department of Oceanography and Fisheries, University Açores, Rua Prof Frederico Machado, 9901-862, Horta, Portugal

    Ana Colaco

Authors
  1. Raju Rajasabapathy
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  2. Chellandi Mohandass
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  3. Jung-Hoon Yoon
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  4. Syed Gulam Dastager
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  5. Qing Liu
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  6. Thi-Nhan Khieu
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  7. Chu Ky Son
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  8. Wen-Jun Li
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  9. Ana Colaco
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Corresponding author

Correspondence to Chellandi Mohandass.

Additional information

The GenBank/EMBL/DDBJ accession number of the 16S rRNA gene sequence of the strain SSW136T is KC534331.

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Rajasabapathy, R., Mohandass, C., Yoon, JH. et al. Nioella nitratireducens gen. nov., sp. nov., a novel member of the family Rhodobacteraceae isolated from Azorean Island. Antonie van Leeuwenhoek 107, 589–595 (2015). https://doi.org/10.1007/s10482-014-0355-5

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  • DOI: https://doi.org/10.1007/s10482-014-0355-5

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