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Streptosporangium subfuscum sp. nov., isolated from the rhizosphere of marigold (Tagetes erecta L.)

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Abstract

A Gram-stain positive, filamentous bacterial strain, designated strain NEAU-TWSJ13T, was isolated from the rhizosphere of a marigold (Tagetes erecta L.) plant collected in Heilongjiang Province, northeast China, and characterized using a polyphasic approach. The strain was observed to form abundant aerial hyphae differentiated into spherical sporangia. 16S rRNA gene sequence similarity studies showed that strain NEAU-TWSJ13T belongs to the genus Streptosporangium, being most closely related to Streptosporangium fragile DSM 43847T (98.6 %). Phylogenetic analysis of the 16S rRNA gene sequence indicated that it formed a phyletic line with S. fragile DSM 43847T, Streptosporangium jomthongense NBRC 110047T (98.4 % 16S rRNA gene similarity) and Streptosporangium violaceochromogenes DSM 43849T (97.6 % 16S rRNA gene similarity). A combination of DNA–DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-TWSJ13T can be distinguished from S. fragile DSM 43847T and S. jomthongense NBRC 110047T. Moreover, strain NEAU-TWSJ13T can also be differentiated from S. violaceochromogenes DSM 43849T and other Streptosporangium species showing high 16S rRNA gene sequence similarity (>98.0 %) by morphological and physiological characteristics. Therefore, it is proposed that strain NEAU-TWSJ13T represents a novel species of the genus Streptosporangium, for which the name Streptosporangium subfuscum sp. nov. is proposed. The type strain is NEAU-TWSJ13T ( = CGMCC 4.7146T = DSM = 46724T).

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Acknowledgments

This work was supported in part by Grants from the National Outstanding Youth Foundation (No. 31225024), the National Key Project for Basic Research (No. 2010CB126102), the National Key Technology R&D Program (No. 2012BAD19B06), the Program for New Century Excellent Talents in University (NCET-11-0953), the National Natural Science Foundation of China (No. 31372006, 31171913, 31071750 and 31471832), the Outstanding Youth Foundation of Heilongjiang Province (JC201201), the Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP), the Science and Technology Research Project of Heilongjiang Provincial Educational Commission (No. 12541001) and the Youth Science Foundation of Heilongjiang Province (No. QC2014C013).

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Authors and Affiliations

  1. School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Ying Zhou, Chongxi Liu, Yuejing Zhang, Chuang Li, Jia Xing, Lianjie Li, Shuyu Zhou, Xiangjing Wang & Wensheng Xiang

  2. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Harbin, 150030, People’s Republic of China

    Wensheng Xiang

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  1. Ying Zhou
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Correspondence to Xiangjing Wang or Wensheng Xiang.

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Zhou, Y., Liu, C., Zhang, Y. et al. Streptosporangium subfuscum sp. nov., isolated from the rhizosphere of marigold (Tagetes erecta L.). Antonie van Leeuwenhoek 106, 1231–1238 (2014). https://doi.org/10.1007/s10482-014-0293-2

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