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Natronomonas gomsonensis sp. nov., isolated from a solar saltern

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Abstract

A halophilic archaeal strain, SA3T, was isolated from sediment of a solar saltern in Gomso Bay, Republic of Korea. Cells of strain SA3T were observed to be coccoid-shaped, to lyse in distilled water, Gram stain-negative and to form red-pigmented colonies. Strain SA3T was found to require at least 18 % (w/v) NaCl for growth. Optimal growth was observed at 24 % (w/v) NaCl and 6 % (w/v) MgCl2. The optimum pH and temperature for growth were determined to be pH 7.0 and 40 °C, respectively, while the strain was found to grow within pH and temperature ranges of 5.5–8.0 and 20–45 °C, respectively. The polar lipids were determined to consist of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, unidentified phosphoglycolipids and unidentified phospholipids. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SA3T was most closely related to the members of the genus Natronomonas, Natronomonas moolapensis JCM 14361T (95.2 %) and Natronomonas pharaonis JCM 8858T (95.1 %). The genomic DNA G+C content (61.8 mol%) determined for strain SA3T was slightly lower than those of N. moolapensis JCM 14361T (63.4 mol%) and N. pharaonis JCM 8858T (64.3 mol%). DNA–DNA hybridization values between N. moolapensis JCM 14361T and N. pharaonis JCM 8858T and strain SA3T were <20 %. Based on phenotypic, chemotaxonomic and phylogenetic properties, we describe a new species of the genus Natronomonas, represented by strain SA3T (=JCM 17867T = KCTC 4088T), for which we propose the name Natronomonas gomsonensis sp. nov.

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Acknowledgments

This study was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MEST) (No. NRF-2013R1A2A2A05006754), the Korea Polar Research Institute (PP12010) and the “Marine and Extreme Genome Research Center Program” of the Ministry of Ministry of Oceans and Fisheries, Republic of Korea.

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Authors and Affiliations

  1. Department of Microbiology, Chungbuk National University, Cheongju, Republic of Korea

    Tae-Yoon Kim, So-Jeong Kim, Soo-Je Park, Jong-Geol Kim, In-Tae Cha, Man-Young Jung, Sang-A Lee & Sung-Keun Rhee

  2. Jeju Center, Korea Basic Science Institute (KBSI), Jeju, 690-756, Republic of Korea

    Seong Woon Roh & Kyung June Yim

  3. Japan Collection of Microorganisms, RIKEN Bio Resource Center, Wako, Saitama, Japan

    Takashi Itoh

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  1. Tae-Yoon Kim
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Correspondence to Sung-Keun Rhee.

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Tae-Yoon Kim and So-Jeong Kim have contributed equally to this work.

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Kim, TY., Kim, SJ., Park, SJ. et al. Natronomonas gomsonensis sp. nov., isolated from a solar saltern. Antonie van Leeuwenhoek 104, 627–635 (2013). https://doi.org/10.1007/s10482-013-9970-9

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