Skip to main content
SpringerLink
Log in

Catching speciation in the act: Metschnikowia bowlesiae sp. nov., a yeast species found in nitidulid beetles of Hawaii and Belize

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

We describe the species Metschnikowia bowlesiae sp. nov. based on the recovery of six isolates from Hawaii and Belize. The species belongs to the Metschnikowia arizonensis subclade of the large-spored Metschnikowia clade. The isolates are haploid and heterothallic. Both Hawaiian strains had the mating type h + and the Belizean strains were h −. Paraphyletic species structures observed in some ribosomal DNA sequence analyses suggest that M. bowlesiae sp. nov. might represent an intermediate stage in a succession of peripatric speciation events from Metschnikowia dekortorum to Metschnikowia similis and might even hybridize with these species. The type of M. bowlesiae sp. nov. is strain UWOPS 04-243x5 (CBS 12940T, NRRL Y-63671) and the allotype is strain UWOPS 12-619.1 (CBS 12939A, NRRL Y-63670).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Clement M, Posada D, Crandall K (2000) TCS: a computer program to estimate gene genealogies. Mol Ecol 9:1657–1660

    Article  CAS  PubMed  Google Scholar 

  • Fidalgo-Jiménez A, Daniel HM, Evrard P, Decock C, Lachance MA (2008) Metschnikowia cubensis sp. nov., a new yeast species isolated from flowers in Cuba. Int J Syst Evol Microbiol 58:2955–2961

    Article  PubMed  Google Scholar 

  • Guzmán B, Lachance MA, Herrera CM (2013) Phylogenetic analysis of the angiosperm–floricolous insect–yeast association: have yeast and angiosperm lineages co-diversified? Mol Phylogenet Evol 68:161–175

    Article  PubMed  Google Scholar 

  • Kurtzman CP, Robnett CJ (2003) Phylogenetic relationships among yeasts of the “Saccharomyces complex” determined from multigene sequence analyses. FEMS Yeast Res 3:417–432

    Article  CAS  PubMed  Google Scholar 

  • Kurtzman CP, Fell JW, Boekhout T, Robert V (2011) Methods for the isolation, phenotypic characterization and maintenance of yeasts. In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts, a taxonomic study, vol 1. Elsevier, Amsterdam, pp 87–110

    Chapter  Google Scholar 

  • Lachance MA (2011) Metschnikowia Kamienski (1899). In: Kurtzman CP, Fell JW, Boekhout T (eds) The Yeasts, a taxonomic study, vol 1. Elsevier, Amsterdam, pp 575–620

    Chapter  Google Scholar 

  • Lachance MA, Bowles JM (2002) Metschnikowia arizonensis and Metschnikowia dekortorum, two new large-spored yeast species associated with floricolous beetles. FEMS Yeast Res 2:81–86

    CAS  PubMed  Google Scholar 

  • Lachance MA, Bowles JM (2004) Metschnikowia similis sp. nov. and Metschnikowia colocasiae sp.nov., two ascomycetous yeasts isolated from Conotelus spp. (Coleoptera: Nitidulidae) in Costa Rica. Stud Mycol 50:69–76

    Google Scholar 

  • Lachance MA, Starmer WT, Phaff HG (1990) Metschnikowia hawaiiensis sp. nov., a heterothallic haploid yeast from hawaiian morning glory and associated drosophilids. Int J Syst Bacteriol 40:415–420

    Article  CAS  PubMed  Google Scholar 

  • Lachance MA, Starmer WT, Bowles JM, Phaff HJ, Rosa CA (2000) Ribosomal DNA, species structure, and biogeography of the cactophilic yeast Clavispora opuntiae. Can J Microbiol 46:195–210

    Article  CAS  PubMed  Google Scholar 

  • Lachance MA, Kaden JE, Phaff HJ, Starmer WT (2001) Phylogenetic structure of the Sporopachydermia cereana species complex. Int J Syst Evol Microbiol 51:237–247

    Article  CAS  PubMed  Google Scholar 

  • Lachance MA, Bowles JM, Starmer WT (2003a) Geography and niche occupancy as determinants of yeast biodiversity: the yeast–insect–morning glory ecosystem of Kīpuka Puaulu, Hawai’i. FEMS Yeast Res 4:104–111

    Google Scholar 

  • Lachance MA, Daniel HM, Meyer W, Prasad GS, Gautam SP, Boundy-Mills K (2003b) The D1/D2 domain of the large-subunit rDNA of the yeast species Clavispora lusitaniae is unusually polymorphic. FEMS Yeast Res 4:253–258

    Article  CAS  PubMed  Google Scholar 

  • Lachance MA, Ewing CP, Bowles JM, Starmer WT (2005) Metschnikowia hamakuensis sp. nov., Metschnikowia kamakouana sp. nov., and Metschnikowia mauinuiana sp. nov., three endemic yeasts from Hawaiian nitidulid beetles. Int J Syst Evol Microbiol 55:1369–1377

    Article  CAS  PubMed  Google Scholar 

  • Marinoni G, Lachance MA (2004) Speciation in the large-spored Metschnikowia clade and establishment of a new species, Metschnikowia borealis comb. nov. FEMS Yeast Res 4:587–596

    Article  CAS  PubMed  Google Scholar 

  • Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542

    Article  PubMed Central  PubMed  Google Scholar 

  • Rosa CA, Lachance MA, Teixeira LCRS, Pimenta RS, Morais PB (2007) Metschnikowia cerradonensis sp. nov., a yeast species isolated from ephemeral flowers and their nitidulid beetles in Brazil. Int J Syst Evol Microbiol 57:161–165

    Article  CAS  PubMed  Google Scholar 

  • Russell J, Zomerdijk JCBM (2005) RNA-polymerase-I-directed rDNA transcription, life and works. Trends Biochem Sci 30:87–96

    Article  CAS  PubMed  Google Scholar 

  • Suchard MA, Weiss RE, Dorman KS, Sinsheimer JS (2003) Inferring spatial phylogenetic variation along nucleotide sequences: a multiple change-point model. J Am Stat Assoc 98:427–437

    Article  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This work was funded by the Natural Science and Engineering Council of Canada. We acknowledge the Granting of research or collecting permits by the Department of Land and Natural Resources of Hawaii, USA, and the Ministry of Natural Resources and the Environment of Belize. Field assistance from Jane Bowles and Curtis Ewing is gratefully acknowledged. The work in Belize was facilitated by Brock Fenton and Mark Howells. We thank Sheila Macfie for her critical reading of the revised manuscript.

Author information

Authors and Affiliations

  1. Department of Biology, University of Western Ontario, London, ON, N6A 5B7, Canada

    Marc-André Lachance & Alexandra N. Fedor

Authors
  1. Marc-André Lachance
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alexandra N. Fedor
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marc-André Lachance.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Online Resource 1

Phylogram of representatives of each ITS-D1/D2 phylotype (Fig. 2) of M. bowlesiae sp. nov., M. dekortorum, and M. similis with M. colocasiae as outgroup. The neighbour-joining analysis was performed on 1946 aligned positions in the ribosomal intergenic spacer (IGS). Gapped positions were deleted on a pair-wise basis. The scale bar shows the number of substitutions, which was the distance measure used in the analysis. Bootstraps from 1,000 replicates are shown. Supplementary material 1 (PDF 184 kb)

Online Resource 2

Phylogram showing the placement of M. bowlesiae sp. nov. in the M. arizonensis subclade. A maximum likelihood analysis was performed on 5439 non-gapped, aligned positions in the ribosomal gene cluster. The distances were fit to the general time reversible model. Bootstraps for 100 iterations are shown. A discrete Γ distribution was used to accommodate rate differences among sites (5 categories, Γ parameter = 0.05). The scale shows the patristic distance. Supplementary material 2 (PDF 102 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lachance, MA., Fedor, A.N. Catching speciation in the act: Metschnikowia bowlesiae sp. nov., a yeast species found in nitidulid beetles of Hawaii and Belize. Antonie van Leeuwenhoek 105, 541–550 (2014). https://doi.org/10.1007/s10482-013-0106-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-013-0106-z

Keywords

Search

Navigation