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Burkholderia caballeronis sp. nov., a nitrogen fixing species isolated from tomato (Lycopersicon esculentum) with the ability to effectively nodulate Phaseolus vulgaris

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Abstract

During a survey of Burkholderia species with potential use in agrobiotechnology, a group of 12 strains was isolated from the rhizosphere and rhizoplane of tomato plants growing in Mexico (Nepantla, Mexico State). A phylogenetic analysis of 16S rRNA gene sequences showed that the strains are related to Burkholderia kururiensis and Burkholderia mimosarum (97.4 and 97.1 %, respectively). However, they induced effective nitrogen-fixing nodules on roots of Phaseolus vulgaris. Based on polyphasic taxonomy, the group of strains represents a novel species for which the name Burkholderia caballeronis sp. nov. is proposed. The type species is TNe-841T (= LMG 26416T = CIP 110324T).

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Acknowledgments

We are indebted to Dr. Euzeby for the etymological construction of the new bacteria species. We are grateful to Marco Antonio Rogel Hernández (Centro de Ciencias Genómicas, UNAM) for technical support. NSF IOS-0747525, IOS 1201735, and the Shanbrom Family Foundation support Burkholderia research in the Hirsch laboratory.

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Authors and Affiliations

  1. Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, 565-A, Cuernavaca, Morelos, Mexico

    Lourdes Martínez-Aguilar, Rafael Díaz Méndez & Jesús Caballero-Mellado

  2. Depto. de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, San Rafael 186, Col. Vicentina, Mexico, DF, Mexico

    Corelly Salazar-Salazar

  3. Department of Molecular, Cell and Developmental Biology, Molecular Biology Institute, University of California, Los Angeles, 621 Charles E. Young Drive South, Los Angeles, CA, 90095-1606, USA

    Ann M. Hirsch

  4. Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prolongación de Carpio Y Plan de Ayala s/n, Col. Santo Tomás, Delegación Miguel Hidalgo, 11340, Mexico, DF, Mexico

    María Soledad Vásquez-Murrieta & Paulina Estrada-de los Santos

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  1. Lourdes Martínez-Aguilar
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  2. Corelly Salazar-Salazar
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  3. Rafael Díaz Méndez
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Correspondence to Paulina Estrada-de los Santos.

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Martínez-Aguilar, L., Salazar-Salazar, C., Méndez, R.D. et al. Burkholderia caballeronis sp. nov., a nitrogen fixing species isolated from tomato (Lycopersicon esculentum) with the ability to effectively nodulate Phaseolus vulgaris . Antonie van Leeuwenhoek 104, 1063–1071 (2013). https://doi.org/10.1007/s10482-013-0028-9

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