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Kineococcus glutineturens sp. nov., isolated from soil in Yunnan, south-west China

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Abstract

An orange-coloured, non-spore-forming, motile and coccus-shaped actinobacterium, designated YIM 75677T, was isolated from a soil sample collected from a dry-hot river valley in Dongchuan county, Yunnan Province, south-west China and its taxonomic position was investigated. Growth of strain YIM 75677T occurred at 12–55 °C, pH 6.0–9.0 and NaCl tolerance up to 2 % (w/v). Cells adhered to agar media and were agglutinated tightly together. The peptidoglycan contained meso-diaminopimelic acid, alanine and glutamic acid. The whole-cell hydrolysates mainly contained glucose, galactose, mannose and ribose. The predominant menaquinone was MK-9 (H2) and the major fatty acids were anteiso-C15:0 and iso-C15:0. Mycolic acids were not present. The DNA G+C content of strain YIM 75677T was 74.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequence comparisons clearly revealed that strain YIM 75677T represents a novel member of the genus Kineococcus and is closely related to Kineococcus xinjiangensis S2-20T (level of similarity, 98.6 %). Meanwhile, the result of DNA–DNA hybridization between strain YIM 75677T and K. xinjiangensis S2-20T demonstrated that this isolate represented a different genomic species in the genus Kineococcus. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain YIM 75677T represents a novel species of the genus Kineococcus, for which the name Kineococcus glutineturens sp. nov. is proposed. The type strain is YIM 75677T (=CCTCC AA 209075T = JCM 18126T).

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Acknowledgments

This research was supported by Science and Technology Innovation Talents Program in Universities of Henan Province of China (HASTIT, No. 2010HASTIT020) and Key Technologies R&D Program of Henan Province of China (112102310335, 112102210106). W-J Li was also supported by ‘Hundred Talents Program’ of the Chinese Academy of Sciences.

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Authors and Affiliations

  1. College of Life Sciences, Henan Normal University, Xinxiang, 453007, People’s Republic of China

    Guo-Xing Nie & Shuai Li

  2. Department of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, 453003, People’s Republic of China

    Hong Ming & Hui-Gen Feng

  3. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Hong Ming, Jing Zhang, Tian-Tian Yu, En-Min Zhou, Shu-Kun Tang & Wen-Jun Li

  4. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, 830011, People’s Republic of China

    Wen-Jun Li

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  1. Guo-Xing Nie
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Correspondence to Shu-Kun Tang or Wen-Jun Li.

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Guo-Xing Nie and Hong Ming contributed equally to this study.

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Nie, GX., Ming, H., Zhang, J. et al. Kineococcus glutineturens sp. nov., isolated from soil in Yunnan, south-west China. Antonie van Leeuwenhoek 102, 239–246 (2012). https://doi.org/10.1007/s10482-012-9731-1

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