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Candida oceani sp. nov., a novel yeast isolated from a Mid-Atlantic Ridge hydrothermal vent (−2300 meters)

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Abstract

A novel species in the genus Candida was obtained from deep-sea hydrothermal fields on the Mid-Atlantic Ridge. Strains Mo39, MARY089 and CBS 5307, respectively, isolated from an unidentified deep-sea coral collected near Rainbow hydrothermal vent, from water samples near Menez Gwen hydrothermal field and from the stomach of a marine fish are considered as a novel taxon. Sequence similarities in the D1/D2 region of the 26S rRNA gene indicated that strains Mo39, MARY089 and CBS 5307 have for closest neighbors Candida spencermartinsiae, Candida taylorii, Candida atmosphaerica and Candida atlantica. The strains, respectively, differ from C. spencermartinsiae, C. taylorii, C. atmosphaerica andCandida atlantica by 4, 4.3, 4.3 and 4.7% in the D1/D2 domain. Strains Mo39, MARY089 and CBS 5307 were differentiated from others by differences in the ability to assimilate d-Gluconate and in the ability to grow at relatively high temperature. Only strain Mo39 displays an optimal growth at 3% sea salts, indicating that this strain is clearly adapted to live in marine conditions. Sequence similarities between strains Mo39, MARY089 and CBS 5307 and related species and differences in the ability to utilize specific carbon compounds revealed that these strains represent a hitherto unknown species. Sexual reproduction was not observed in strains Mo39, MARY089 and CBS 5307. An anamorphic name Candida oceani sp. nov. is proposed for the type strain Mo39T (= CBS 11857T = DSM 23777T) and the two other strains MARY089 and CBS 5307. To our knowledge, this is the first description of a micro-eukaryotic organism including a strain isolated from a deep-sea coral near a hydrothermal ecosystem.

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Acknowledgments

We thank the chief scientist of the MoMARDREAM-Naut cruise, pilots and support crews of oceanographic vessels and Deep Submergence Vehicles of Ifremer. We greatly acknowledge Justine Amborski for valuable help for latin diagnosis. We thank all members of the GDR Ecchis for discussions and suggestions. We would like to thank the editor and the anonymous reviewers who have provided helpful comments on the refinement of this manuscript. We also thank ANR Deep-Oases and French Research Ministry for financial support.

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Authors and Affiliations

  1. Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (EA3882), IFR 148, Université Européenne de Bretagne, Université de Brest, ESMISAB, Technopole Brest-Iroise, 29280, Plouzané, France

    Gaëtan Burgaud, Danielle Arzur & Georges Barbier

  2. Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal

    José Paulo Sampaio

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  1. Gaëtan Burgaud
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  2. Danielle Arzur
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  3. José Paulo Sampaio
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  4. Georges Barbier
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Correspondence to Gaëtan Burgaud.

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Burgaud, G., Arzur, D., Sampaio, J.P. et al. Candida oceani sp. nov., a novel yeast isolated from a Mid-Atlantic Ridge hydrothermal vent (−2300 meters). Antonie van Leeuwenhoek 100, 75–82 (2011). https://doi.org/10.1007/s10482-011-9566-1

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