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Rubellimicrobium roseum sp. nov., a Gram-negative bacterium isolated from the forest soil sample

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Abstract

A novel pink-coloured, non-spore-forming, non-motile, Gram-negative bacterium, designated YIM 48858T, is described by using a polyphasic approach. The strain can grow at pH 6.5–9 (optimum at pH 7) and 25–30°C (optimum at 28°C). NaCl is not required for its growth. Positive for oxidase and catalase. Urease activity, nitrate reduction, starch and Tween 80 tests are negative reaction. 16S rRNA gene sequence similarity studies showed that strain YIM 48858T is a member of the genus Rubellimicrobium, with similarities of 96.3, 95.7 and 95.5% to Rubellimicrobium mesophilum MSL-20T, Rubellimicrobium aerolatum 5715S-9T and Rubellimicrobium thermophilum DSM 16684T, respectively. Q-10 was the predominant respiratory ubiquinone as in the other members of the genus Rubellimicrobium. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphoglycolipid, glycolipid and the major fatty acids were C18:1 ω7c, C16:0 and C10:0 3-OH, which are very different from the valid published species. The DNA G + C content was 67.7 mol%. Both phylogenetic and chemotaxonomic evidence supports that YIM 48858T is a novel species of the genus Rubellimicrobium, for which the name Rubellimicrobium roseum sp. nov. is proposed. The type strain is YIM 48858T (=CCTCC AA 208029T =KCTC 23202T).

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (No. 30900002 and No. 30560001), International Cooperative Key Project of Ministry of Science and Technology (2006DFA33550). Authors are grateful to Yong-Xia Wang, Yun Wang and Yun Chen for their help in technique.

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Authors and Affiliations

  1. College of Resources and Environment, Northwest A & F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Yan-Ru Cao, Wen-Xiang He & Quan-Hong Xue

  2. The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Yan-Ru Cao, Yi Jiang, Qian Wang, Shu-Kun Tang, Li-Hua Xu & Cheng-Lin Jiang

  3. The National Engineering Research Center for Microbial Pharmaceutical, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Yan-Ru Cao, Yi Jiang, Qian Wang, Shu-Kun Tang, Li-Hua Xu & Cheng-Lin Jiang

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  1. Yan-Ru Cao
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  2. Yi Jiang
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  3. Qian Wang
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  4. Shu-Kun Tang
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Correspondence to Yi Jiang.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM 48858T is GU109478.

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Cao, YR., Jiang, Y., Wang, Q. et al. Rubellimicrobium roseum sp. nov., a Gram-negative bacterium isolated from the forest soil sample. Antonie van Leeuwenhoek 98, 389–394 (2010). https://doi.org/10.1007/s10482-010-9452-2

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