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A study on the effects of food quantity and quality on glutathione S-transferase (GST) activity and growth rate parameters of Daphnia carinata varying in age

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Abstract

The adverse influences of insufficient food and toxins on Daphnia carinata’s body growth, reproduction and tolerance were investigated in the laboratory. Different concentrations of Scendesmus obliquus and a mixture of S. obliquus and microcystin (MC)-containing Microcystis aeruginosa PCC7820 were used to feed D. carinata. Glutathione S-transferase (GST) activity towards five chemical compounds (substrates) was measured and used as an indicator of their tolerance. Body growth rate and clutch size of D. carinata decreased with declined concentration or decreased proportion of S. obliquus in the diet. GST activity decreased with ageing in D. carinata. However, GST activity to several chemical compounds increased when food quantity or food quality decreased. Adult D. carinata had a lower GST activity towards p-nitrophenenzyl chloride (PNBC) than juveniles and exhibited a sharp decline (P < 0.001) in GST activity towards PNBC as the animals aged. It is suggested that the age-specific decrease in GST activity is a possible mechanism for the high morality of adult D. carinata in the summer of eutrophic lakes.

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Acknowledgments

I am much indebted to Prof. Leblanc for providing me with a special custom-made version of the methods for analysing GST activities. I would like to thank Prof. Kong for discussing and correcting the manuscript. Min Zhang for greatly improving the English of an earlier draft of the manuscript and two anonymous referees, whose critical comment led to considerable improvement. This study was supported by the National Basic Research Programme of China (973 Programme, Grant No. 2008CB418101).

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  1. Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Donghu South Road 7, 430072, Wuhan, People’s Republic of China

    Nichun Guo & Ping Xie

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  1. Nichun Guo
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  2. Ping Xie
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Correspondence to Ping Xie.

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Handling Editor: R. D. Gulati.

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Guo, N., Xie, P. A study on the effects of food quantity and quality on glutathione S-transferase (GST) activity and growth rate parameters of Daphnia carinata varying in age. Aquat Ecol 45, 63–73 (2011). https://doi.org/10.1007/s10452-010-9324-x

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  • DOI: https://doi.org/10.1007/s10452-010-9324-x

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