Abstract
This work presents an analysis on the suitability of the Simulated Moving Bed (and pseudo-SMB) technology on separating the mixture obtained from the lactose oxidation catalyzed by glucose-fructose oxidoreductase and glucono-α-lactonase enzymes. These enzymes from the Zymomonas mobilis bacteria are able to oxidize lactose in presence of fructose to its respective organic acid—lactobionic acid—and sorbitol. Some alternative arrays of chromatographic systems, as fixed bed column, SMB unit, 4 section pseudo-SMB, have been explored to separate the multi-component mixture, in such way to make possible the product recovery and the recycle of substrates to the enzymatic reactor. This study involved the definition of appropriate operating conditions and the prediction of the performance of the separation units, or arrangement of units, through modeling and simulations tools. To define the proper operating conditions, inequalities from equilibrium theory and the concept of the separation volume analysis have been considered. In this analysis, equilibrium and kinetic parameters for the compounds adsorbing on DOWEX 50W-X4 resin, in K+ and Ca+2 ion-loadings, have been obtained from chromatographic methods (pulse and adsorption-desorption techniques). The enzymatic kinetic of production of lactobionic acid and sorbitol using permeabilized cells of Z. mobilis is shown. The strategy of keeping the highest value of reaction rate by the integration of a chromatographic system proved to be viable when it was found the feasibility to apply the SMB system in cascade.
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References
Ando, M., Tanimura, M., Tamura, M.: Method of chromatographic separation. U.S. Patent 4,970,002 (1990)
Azevedo, D.C.S.: Separation/reaction in simulated moving bed. Ph.D. thesis, University of Porto, Porto, Portugal (2001)
Azevedo, D.C.S., Rodrigues, A.E.: Design of a simulated moving bed in the presence of mass-transfer resistances. AIChE J. 45(5), 956–966 (1999)
Bae, Y.S., Moon, J.H., Lee, C.H.: Effects of feed concentration on the startup and performance behaviors of simulated moving bed chromatography. Ind. Eng. Chem. Res. 45(2), 777–790 (2006)
Baminger, U., Ludwig, R., Galhaup, C., Leitner, C., Kulbe, K., Haltrich, D.: Continuous enzymatic regeneration of redox mediators used in bio-transformation reactions employing flavoproteins. J. Mol. Catal. B 11, 541–550 (2001)
Beste, Y.A., Arlt, W.: Side-stream simulated moving-bed chromatography for multicomponent separation. Chem. Eng. Technol. 25(10), 956–962 (2002)
Borges da Silva, E.A., Rodrigues, A.E.: Design of chromatographic multicomponent separation by a pseudo-simulated moving bed. AIChE J. 52(11), 3794–3812 (2006)
Borges da Silva, E.A., Rodrigues, A.E.: Design methodology and performance analysis of a pseudo-simulated moving bed for ternary separation. Sep. Sci. Technol. 43(3), 533–566 (2008)
Bowen, W.H.: Food components and caries. Adv. Dent. Res. 8(2), 215–220 (1994)
Cen, P., Tsao, G.T.: Recent advances in the simultaneous bioreaction and product separation processes. Sep. Purif. Technol. 3, 58–75 (1993)
Chiang, A.S.T.: Continuous chromatographic process based on SMB technology. AIChE J. 44(8), 1930–1932 (1998)
Coté, A., Brown, W.A., Cameron, D., van Walsum, P.: Hydrolysis of lactose in whey permeate for subsequent fermentation to ethanol. J. Dairy Sci. 87, 1608–1620 (2004)
Druliolle, H., Kokoh, K.B., Beden, B.: Selective oxidation of lactose to lactobionic acid on lead-adatoms modified platinum-electrodes in Na2CO3+NaHCO3 buffered medium. J. Electroanal. Chem. 385(1), 77–83 (1995)
Erzinger, G.S., Silveira, M.M., Lopes da Costa, J.P.C., Vitolo, M., Jonas, R.: Activity of glucose-fructose oxidoreductase in fresh and permeabilised cells of Zymomonas mobilis grown in different glucose concentrations. Braz. J. Microbiol. 34(4), 329–333 (2003)
Fox, P.F., McSweeney, P.L.H.: Lactose. In: Fox, P.F., McSweeney, P.L.H. (eds.) Dairy Chemistry & Biochemistry, pp. 21–66. Kluwer Academic/Plenum, New York (1998)
Ganetsos, G., Barker, P.E., Ajongwen, J.N.: Batch and continuous chromatographic systems as combined bioreactors-separators. In: Ganetsos, G., Barker, P.E. (eds.) Preparative and Production Scale Chromatography. Dekker, New York (1993)
Heuer, C., Kniep, H., Falk, T., Seidel-Morgenstern, A.: Comparison of various process engineering concepts of preparative liquid chromatography. Chem. Ing. Tech. 69(11), 1535 (1997)
Hur, J.S., Wankat, P.C.: Two-zone SMB/chromatography for center-cut separation from ternary mixtures: Linear isotherm systems. Ind. Eng. Chem. Res. 45(4), 1426–1433 (2006)
Jupke, A., Epping, A., Schmidt-Traub, H.: Optimal design of batch and simulation moving bed chromatographic separation processes. J. Chromatogr. A 944(1–2), 93–117 (2002)
Kawajiri, Y., Biegler, L.T.: Optimization strategies for simulated moving bed and PowerFeed processes. AIChE J. 52(4), 1343–1350 (2006)
Kim, J.K., Wankat, P.C.: Designs of simulated-moving-bed cascades for quaternary separations. Ind. Eng. Chem. Res. 43(4), 1071–1080 (2004)
Kokoh, K.B., Alonso-Vante, N.: Electrocatalytic oxidation of lactose on gold nanoparticle modified carbon in carbonate buffer. J. Appl. Electrochem. 36(2), 147–151 (2006)
Kurup, A.S., Hidajat, K., Ray, A.K.: Optimal operation of a pseudo-SMB process for ternary separation under non-ideal conditions. Sep. Purif. Technol. 51(3), 387–403 (2006)
Maliska, C.R.: Transferência de Calor e Mecânica dos Fluidos Computacional. LTC—Livros Técnicos e Científicos S.A., Rio de Janeiro (1995)
Malvessi, E., Concatto, K., Silveira, M.M.: Biotransformação de frutose e lactose em sorbitol e ácido lactobiônico por células de Zymomonas mobilis. In: Proceeding of 5 ° Brazillian seminary of Enzymatic Technology—Enzitec, 2002, Brasília (DF), Brasil (2002)
Malvessi, E., Concatto, K., Carra, S., Silveira, M.M.: Formulation of medium for growth and production of ethanol and intracellular enzymes by Zymomonas mobilis. Braz. Arch. Biol. Technol. 49, 139–144 (2006)
Marwaha, S.S., Kennedy, J.F.: Whey-pollution problem and potential utilization. Int. J. Food Sci. Technol. 23(4), 323–336 (1988)
Mata, V.G., Rodrigues, A.E.: Separation of ternary mixtures by pseudo-simulated moving bed chromatography. J. Chromatogr. A 939(1–2), 23–40 (2001)
Mihlbachler, K., Fricke, J., Yun, T., Seidel-Morgenstern, A., Schmidt-Traub, H., Guiochon, G.: Effect of the homogeneity of the column set on the performance of a simulated moving bed unit. I. Theory. J. Chromatogr. A 908(1–2), 49–70 (2001)
Murakami, H., Kawano, J., Yoshizumi, H., Nakano, H., Kitahata, S.: Screening of lactobionic acid producing microorganisms. J. Appl. Glycosci. 49, 469–477 (2002)
Navarro, A., Caruel, H., Rigal, L., Phemius, P.: Continuous chromatographic separation process: Simulated moving bed allowing simultaneous withdrawal of three fractions. J. Chromatogr. A 770(1–2), 39–50 (1997)
Nordkvist, M., Nielsen, P.M., Villadsen, J.: Oxidation of lactose to lactobionic acid by a Microdochium nivale carbohydrate oxidase: Kinetics and operational stability. Biotechnol. Bioeng. 97(4), 694–707 (2007)
Pais, L.S., Loureiro, J.M., Rodrigues, A.E.: Modeling strategies for enantiomers separation by SMB chromatography. AIChE J. 44(3), 561–569 (1998)
Pedruzzi, I., Malvessi, E., Mata, V.G., Silva, E.A.B., Silveira, M.M., Rodrigues, A.E.: Quantification of lactobionic acid and sorbitol from enzymatic reaction of fructose and lactose by high-performance liquid chromatography. J. Chromatogr. A 1145(1–2), 128–132 (2007)
Pedruzzi, I., da Silva, E.A.B., Rodrigues, A.E.: Selection of resins equilibrium and sorption kinetics of lactobionic acid, fructose, lactose and sorbitol. Sep. Purif. Technol. 63(3), 600–611 (2008)
Ruthven, D.M., Ching, C.B.: Counter current and simulated counter-current adsorption separation processes. Chem. Eng. Sci. 44(5), 1011–1038 (1989)
Satory, M., Furlinger, M., Haltrich, D., Kulbe, K.D., Pittner, F., Nidetzky, B.: Continuous enzymatic production of lactobionic acid using glucose-fructose oxidoreductase in an ultrafiltration membrane reactor. Biotechnol. Lett. 19(12), 1205–1208 (1997)
Sayama, K., Kamada, T., Oikawa, S., Masuda, T.: Production of raffinose—a new by-product of the beet sugar-industry. Zucker-Ind. 117(11), 893–898 (1992)
Silveira, M., Wisbeck, E., Lemmel, C., Erzinger, G., Lopes da Costa, J., Bertasso, M., Jonas, R.: Bioconversion of glucose and fructose to sorbitol and gluconic acid by untreated cells of Zymomonas mobilis. J. Biotechnol. 75, 99–103 (1999)
Storti, G., Mazzotti, M., Morbidelli, M., Carra, S.: Robust design of binary counter-current adsorption separation processes. AIChE J. 39(3), 471–492 (1993)
Strube, J., Haumreisser, S., Schmidt-Traub, H., Schulte, M., Ditz, R.: Comparison of batch elution and continuous simulated moving bed chromatography. Org. Process Res. Dev. 2(5), 305–319 (1998)
Sumimoto, R., Kamada, N.: Lactobionate as the most important component in UW solution for liver preservation. Transplant. Proc. 22(5), 2198–2199 (1990)
Wang, X., Ching, C.B.: Chiral separation of beta-blocker drug (nadolol) by five-zone simulated moving bed chromatography. Chem. Eng. Sci. 60(5), 1337–1347 (2005)
Wankat, P.C.: Simulated moving bed cascades for ternary separations. Ind. Eng. Chem. Res. 40(26), 6185–6193 (2001)
Wooley, R., Ma, Z., Wang, N.H.L.: A nine-zone simulating moving bed for the recovery of glucose and xylose from biomass hydrolyzate. Ind. Eng. Chem. Res. 37(9), 3699–3709 (1998)
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Borges da Silva, E.A., Pedruzzi, I. & Rodrigues, A.E. Simulated moving bed technology to improve the yield of the biotechnological production of lactobionic acid and sorbitol. Adsorption 17, 145–158 (2011). https://doi.org/10.1007/s10450-010-9304-4
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DOI: https://doi.org/10.1007/s10450-010-9304-4