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Effects of Hydroxyapatite and Biostite® on Osteogenic Induction of hMSC

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Abstract

When isolated from the iliac crest human mesenchymal stem cells (hMSC) differentiate into osteoblast-like cells with appropriate stimulation in culture. This in vitro study tested the hypothesis that Biostite® and hydroxyapatite (HA) affect proliferation and differentiation of hMSC into osteoblastic cells. Cell proliferation was determined by measuring 3H-thymidine incorporation into DNA and typical markers of osteoblastic phenotype were determined by RT-PCR assay. No differences emerged in cell proliferation cultures with Biostite® or hydroxyapatite (HA), but gene expression analysis revealed higher expression of collagen, alkaline phosphatase (ALP), osteopontin and bone sialoprotein (BSP) in the presence of Biostite®. TGFβ2 production, as assessed by an Elisa kit, and Runx2 expression by RT-PCR, were greater in Biostite cultures, suggesting Biostite® provides a better environment for hMSC differentiation into osteoblasts and is, potentially, a more promising bone-filling material than HA.

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Acknowledgments

We thank Dr. G.A. Boyd for help with the English translation. Moreover we thank the Fondazione Cassa di Risparmio of Perugia for providing the instruments for in vitro experiments.

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Authors and Affiliations

  1. Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy

    Lorella Marinucci, Stefania Balloni, Ennio Becchetti & Paola Locci

  2. Department of Dermatology and Plastic Surgery, University La Sapienza, Rome, Italy

    Giovanni Bistoni

  3. Piazza B. Buozzi 36, Ospedalicchio, Perugia, Italy

    Edoardo Maria Calvi

  4. Forensic Medicine Institute, University of Perugia, Perugia, Italy

    Eleonora Lumare

  5. via Beethoven 1, Ellera di Corciano, Perugia, Italy

    Filippo Ederli

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  1. Lorella Marinucci
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  2. Stefania Balloni
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  4. Giovanni Bistoni
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Correspondence to Paola Locci.

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Associate Editor Larry V. McIntire oversaw the review of this article.

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Marinucci, L., Balloni, S., Becchetti, E. et al. Effects of Hydroxyapatite and Biostite® on Osteogenic Induction of hMSC. Ann Biomed Eng 38, 640–648 (2010). https://doi.org/10.1007/s10439-009-9898-4

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  • DOI: https://doi.org/10.1007/s10439-009-9898-4

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