Abstract
We verified the clinical–epidemiological, microbiological, and molecular aspects of feline sporotrichosis in Espírito Santo, Brazil, as well as demonstrated the degree of reliability of the cytopathological examination in diagnosis, when compared to fungal culture. From March 2019 to April 2020, 154 suspicious cats were evaluated by collecting data and biological samples. The Cohen's kappa coefficient and the Chi-square independence defined the degree of agreement and the accuracy parameters, respectively. The interobserver agreement was high (Kappa coefficient = 0.96). Sensitivity, specificity, predictive (positive and negative), and accuracy values were 95.0, 97.0, 99.1, 84.2, and 95.5%, respectively. All isolates evaluated were identified as Sporothrix brasiliensis by species-specific PCR. A digital image bank (Padlet) was created with different types of clinical forms and positive slide profiles. We concluded that the cytopathological technique used demonstrated a high degree of reliability. We report an overwhelming occurrence of S. brasiliensis during epizooties in ES.
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Funding
Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP 2017/27265–5, Fundação de Amparo à Pesquisa do Espírito Santo, FAPES/ES 111/2017, National Council for Science and Technological Development, CNPq 304902/2020–9., CNPq 433276/2018–5.
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The study was performed in strict accordance with recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and approved by the Institutional Ethics in Research Committee on the use of animals (CEUA) of the Federal University of Espírito Santo (protocol number 15/2019).
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Below is the link to the electronic supplementary material.Supplementary Figure 1. Agarose gel electrophoresis shows successful amplification with Sporothrix brasiliensis species-specific primer sets (Sbra-F and Sbra-R) and templates as previously described (Rodrigues et al., 2015). Amplicons were sized by comparison with bands of known size from the GeneRuler 100 bp Plus DNA Ladder (Marker) (Thermo Fisher 25 Scientific, USA). C+(Ss54): Sporothrix brasiliensis positive control; C-(Ss118): Sporothrix schenckii negative control. NTC: no template control.
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Rediguieri, B.C., da Cruz Bahiense, I., de Carvalho, J.A. et al. Clinical, Epidemiological, and Epizootic Features of Sporothrix brasiliensis in Espírito Santo, Brazil. EcoHealth 19, 124–134 (2022). https://doi.org/10.1007/s10393-022-01578-8
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DOI: https://doi.org/10.1007/s10393-022-01578-8