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Broad Distribution of Ranavirus in Free-Ranging Rana dybowskii in Heilongjiang, China

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Abstract

Ranaviruses have been associated with die-offs in cultured amphibians in China, but their presence in wild amphibians has not yet been assessed. We sampled free-ranging Rana dybowskii at seven sites throughout Heilongjiang Province to determine the presence and prevalence of ranaviruses in this region. Our results revealed an overall infection prevalence of 5.7% (18/315) for adults and 42.5% (51/120) for tadpoles by PCR. PCR-amplified product showed a high degree of homology with several members of the Iridoviridae, mostly with those belonging to the genus Ranavirus. The results indicate that ranaviruses are broadly distributed throughout Heilongjiang Province and could have important implications for the health of native wildlife. Additional sampling and management strategies should be urgently adopted to address the prevalence and health consequences of ranaviruses throughout China.

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References

  • Brunner JL, Schock DM, Davidson EW, Collins JP (2004) Intraspecific reservoirs: Complex life history and the persistence of a lethal Ranavirus. Ecology 85:560-566

    Article  Google Scholar 

  • Brunner JL, Richards K, Collins JP (2005) Dose and host characteristics influence virulence of ranavirus infections. Oecologia 144:399–406

    Article  Google Scholar 

  • Carey C (1993) Hypothesis concerning the causes of the disappearance of boreal toads from the mountains of Colorado. Conservation Biology 7:355-362

    Article  Google Scholar 

  • Carey C, Cohen N, Rollins-Smith L (1999) Amphibian declines: an immunologic perspective. Developmental and Comparative Immunology 23:459-472

    Article  CAS  Google Scholar 

  • Chinchar VG (2002) Ranaviruses (family Iridoviridae): emerging cold-blooded killers. Archives of Virology 147:447–470

    Article  CAS  Google Scholar 

  • Daszak P, Berger L, Cunningham AA, Hyatt AD, Green DE, Speare R (1999) Emerging infectious diseases and amphibian population declines. Emerging Infectious Diseases 5:735–748

    Article  CAS  Google Scholar 

  • Daszak P, Cunningham AA, Hyatt AD (2003) Infectious disease and amphibian population declines. Diversity and Distributions 9:141–150

    Article  Google Scholar 

  • Galli L, Pereira A, Márquez A, Mazzoni R (2006) Ranavirus detection by PCR in cultured tadpoles (Rana catesbeiana Shaw, 1802) from South America. Aquaculture 257:78-82

    Article  CAS  Google Scholar 

  • Gantress J, Maniero GD, Cohen N, Robert J (2003) Development and characterization of a model system to study amphibian immune responses to iridoviruses. Virology 311:254–262

    Article  CAS  Google Scholar 

  • Go J, Lancaster M, Deece K, Dhungyel O, Whittington R (2006) The molecular epidemiology of iridovirus in Murray cod (Maccullochella peelii peelii) and dwarf gourami (Colisa lalia) from distant biogeographical regions suggests a link between trade in ornamental fish and emerging iridoviral diseases. Molecular and Cellular Probes 20:212–222

    Article  CAS  Google Scholar 

  • Gray MJ, Miller DL, Schmutzer AC, Baldwin CA (2007) Frog virus 3 prevalence in tadpole populations inhabiting cattle-access and non-access wetlands in Tennessee, USA. Diseases of Aquatic Organisms 77:97–103

    Article  Google Scholar 

  • Green DE, Converse KA, Schrader AK (2002) Epizootiology of sixty-four amphibian morbidity and mortality events in the USA, 1996–2001. Annals of New York Academy Science 969:323–339

    Article  Google Scholar 

  • Greer AL, Briggs CJ, Collins JP (2008) Testing a key assumption of host-pathogen theory: density and disease transmission. Oikos 117:1667-1673

    Google Scholar 

  • Greer AL, Collins JP (2008) Habitat fragmentation as a result of biotic and abiotic factors controls pathogen transmission throughout a host population. Journal of Animal Ecology 77:364–369

    Article  Google Scholar 

  • Harp EM, Petranka JW (2006) Ranavirus in wood frogs (Rana sylvatica): potential sources of transmission within and between ponds. Journal of Wildlife Diseases 42:307–318

    Google Scholar 

  • He JG, Lü L, Deng M, He HH, Weng SP, Wang XH, et al. (2002) Sequence analysis of the complete genome of an iridovirus isolated from the tiger frog. Virology 292:185–197

    Article  CAS  Google Scholar 

  • Jancovich JK, Mao J, Chinchar VG, Wyatt C, Case ST, Kumar S, et al. (2003) Genomic sequence of a ranavirus (family Iridoviridae) associated with salamander mortalities in North America. Virology 316:90–103

    Article  CAS  Google Scholar 

  • Kanchanakhan S (1998) An ulcerative disease of the cultured tiger frog, Rana tigrina, in Thailand: Virological examination. Aquatic Animal Health Research Institute Newsletter 7:1-2

    Google Scholar 

  • Kusrini MD, Skerratt LF, Garland S, Berger L, Endarwin W (2008) Chytridiomycosis in frogs of Mount Gede Pangrango, Indonesia. Diseases of Aquatic Organisms 82:187–194

    Article  CAS  Google Scholar 

  • Lips KR, Brem F, Brenes R, Reeve JD, Alford RA, Voyles J, et al. (2006) Emerging infectious disease and the loss of biodiversity in a neotropical amphibian community. The National Academy Science of the USA 103: 3165–3170

    Article  CAS  Google Scholar 

  • Liu X, Zhang W, Yu BC, Liu WS (2007) Investigation on Rana chensinensis Resources in the Three Northeastern Provinces of China. Forest Resources Management 3:82-85

    Google Scholar 

  • Mao J, Hedrick RP, Chinchar VG (1997) Molecular characterization, sequence analysis, and taxonomic position of newly isolated fish iridoviruses. Virology 229:212–220

    Article  CAS  Google Scholar 

  • Mazzoni R, de Mesquita AJ, Fleury LFF, de Brito WMED, Nunes IA, Robert J, et al. (2009) Mass mortality associated with a frog virus 3-like Ranavirus infection in farmed tadpoles Rana catesbeiana from Brazil. Diseases of Aquatic Organisms 86:181-191

    Article  CAS  Google Scholar 

  • McLeod DS, Sheridan JA, Jiraungkoorskul W, Khonsue W (2008) A survey for chytrid fungus in Thai amphibians. The Raffles Bulletin of Zoology 56:199–204

    Google Scholar 

  • Miller DL, Rajeev S, Gray MJ, Baldwin CA (2007) Frog Virus 3 Infection, Cultured American Bullfrogs. Emerging Infectious Diseases 13:342-343

    Article  Google Scholar 

  • Pasmans F, Blahak S, Martel A, Pantchev N, Zwart P (2008) Ranavirus-associated mass mortality in imported red tailed knobby newts (Tylototriton kweichowensis): A case report. Veterinary Journal 176:257–259

    Article  Google Scholar 

  • Pearman PB, Garner TW, Straub M, Greber UF (2004) Response of the Italian agile frog (Rana latastei) to a Ranavirus, frog virus 3: a model for viral emergence in naïve populations. Journal of Wildlife Diseases 40:660–669

    Google Scholar 

  • Picco AM, Collins JP (2008) Amphibian commerce as a likely source of pathogen pollution. Conservation Biology 22:1582–1589

    Article  Google Scholar 

  • Rachowicz LJ, Knapp RA, Morgan JA, Stice MJ, Vredenburg VT, Parker JM, et al. (2006) Emerging infectious disease as a proximate cause of amphibian mass mortality. Ecology 87:1671–1683

    Article  Google Scholar 

  • Rojas S, Richards K, Jancovich JK, Davidson EW (2005) Influence of temperature on Ranavirus infection in larval salamanders Ambystoma tigrinum. Diseases of Aquatic Organisms 63:95-100

    Article  Google Scholar 

  • Rowley JJ, Chan SK, Tang WS, Speare R, Skerratt LF, Alford RA, et al. (2007) Survey for the amphibian chytrid Batrachochytrium dendrobatidis in Hong Kong in native amphibians and in the international amphibian trade. Diseases of Aquatic Organisms 78:87–95

    Article  Google Scholar 

  • Schloegel LM, Hero J-M, Berger L, Speare R, McDonald K, Daszak P (2006) The decline of the sharp-snouted day frog (Taudactylus acutirostris): the first documented case of extinction by infection in a free-ranging wildlife species? EcoHealth 3:35-40

    Article  Google Scholar 

  • Schloegel LM, Picco AM, Kilpatrick AM, Davies AJ, Hyatt AD, Daszak P (2009) Magnitude of the U.S. trade in amphibians and presence of Batrachochytrium dendrobatidis and ranavirus infection in imported North American bullfrogs (Rana catesbeiana). Biological Conservation 142:1420-1426

    Article  Google Scholar 

  • Schloegel LM, Ferreira CM, James TY, Hipolito M, Longcore JE, Hyatt AD, et al. (2010) The North American bullfrog as a reservoir for the spread of Batrachochytrium dendrobatidis in Brazil. Animal Conservation doi:10.1111/j.1469-1795.2009.00307.x

  • Stuart SN, Chanson JS, Cox NA, Young BE, Rodrigues AS, Fishman DL, et al. (2004) Status and trends of amphibian declines and extinctions worldwide. Science 306:1783–1786

    Article  CAS  Google Scholar 

  • St-Amour V, Wong WM, Garner TW, Lesbarrères D (2008) Anthropogenic Influence on Prevalence of 2 Amphibian Pathogens. Emerging Infectious Diseases 14:1175-1176

    Article  Google Scholar 

  • Une Y, Kadekaru S, Tamukai K, Goka K, Kuroki T (2008) First report of spontaneous chytridiomycosis in frogs in Asia. Diseases of Aquatic Organisms 82:157–160

    Article  Google Scholar 

  • Wei Y, Xu K, Zhu DZ, Chen XF, Wang XL (2010) Early-spring survey for Batrachochytrium dendrobatidis in wild Rana dybowskii in Heilongjiang Province, China. Diseases of Aquatic Organisms doi:10.3354/dao02172

  • Weng SP, He JG, Wang XH, Lu L, Deng M, Chan SM (2002) Outbreaks of an iridovirus disease in cultured tiger frog, Rana tigrina rugulosa, in southern China. Journal of Fish Diseases 25:423-427

    Article  Google Scholar 

  • Zhang QY, Xiao F, Li ZQ, Gui JF, Mao J, Chinchar VG (2001) Characterization of an Iridovirus from the cultured pig frog Rana grylio with lethal syndrome. Diseases of Aquatic Organisms 48:27-36

    Article  CAS  Google Scholar 

  • Zhao EM (1998) China red data book of endangered animals (amphibians and reptiles). Beijing: Science Press, p 73

    Google Scholar 

  • Zupanovic Z, Musso C, Lopez G, Louriero CL, Hyatt AD, Hengstberger S, et al. (1998) Isolation and characterization of iridoviruses from the giant toad Bufo marinus in Venezuela. Diseases of Aquatic Organisms 33:1-9

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Supported by China Postdoctoral Science Foundation (Code: 20080430872).

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Authors and Affiliations

  1. Wildlife Resource College, Northeast Forestry University, Ministry of Education of P.R. China, 26 Hexing Road, Xiangfang District, Harbin, Heilongjiang province, 150040, People’s Republic of China

    Kai Xu, Dong-Ze Zhu, Ying Wei, Xiao-Feng Chen & Xiao-Long Wang

  2. Wildlife Trust, 460 West 34th Street, New York, NY, 10001, USA

    Lisa M. Schloegel

Authors
  1. Kai Xu
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  2. Dong-Ze Zhu
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  3. Ying Wei
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  4. Lisa M. Schloegel
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  5. Xiao-Feng Chen
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  6. Xiao-Long Wang
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Correspondence to Xiao-Long Wang.

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Xu, K., Zhu, DZ., Wei, Y. et al. Broad Distribution of Ranavirus in Free-Ranging Rana dybowskii in Heilongjiang, China. EcoHealth 7, 18–23 (2010). https://doi.org/10.1007/s10393-010-0289-y

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  • DOI: https://doi.org/10.1007/s10393-010-0289-y

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