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A comparative analysis of TLR5 polymorphism and clinical parameters in typhoid patients and asymptomatic typhoid carriers

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Abstract

Aim

Typhoid fever is a serious health problem in tropical and developing countries. Although asymptomatic typhoid carriers show no clinical signs, they can spread infection to others. This study investigated the Toll like receptor 5 (TLR5) polymorphism and various clinical parameters in typhoid patients and asymptomatic typhoid carriers.

Subjects and methods

TLR5 gene was amplified in typhoid patients (n = 15), asymptomatic carriers (n = 10) and healthy control subjects (n = 10) by polymerase chain reaction (PCR). A restriction fragment length polymorphism (RFLP) analysis of TLR5 gene was performed with restriction enzyme (Ddel). Clinical parameters such as total leukocyte, platelets, C-reactive protein (CRP), and adenosine de-aminase (ADA) were estimated. A correlative analysis of TLR5 gene polymorphism and clinical parameters was performed.

Results

Typhoid fever patients had significantly (p < 0.05) reduced total leukocyte and platelet counts when compared to asymptomatic carriers and control subjects. Typhoid patients showed significantly (p < 0.05) reduced ADA activity, while elevated CRP level when compared to carriers and controls. TLR5 gene was amplified in all individuals, but only two typhoid patients showed TLR5 gene polymorphism (TLR5 392STOP). There was no significant correlation between TLR5 gene polymorphism and clinical parameters studied in typhoid patients.

Conclusion

The TLR5 gene polymorphism seems to be not associated with susceptibility to typhoid fever.

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Abbreviations

TLR5 :

Toll like receptor 5

RFLP:

Restriction fragment length polymorphism

PCR:

Polymerase chain reaction

CRP:

C-reactive protein

ADA:

Adenosine de-aminase

SNPs:

Single-nucleotide polymorphisms

TCS:

Total clinical scores

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Acknowledgements

Authors are thankful to Muthayammal College of Arts and Sciences for providing the facilities through the research scholars grant to carry out this study. Authors are also thankful to Ms. Helen England for her critical evaluation in the preparation of the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Medical Microbiology, School of Medicine, Health and Medical Science College, Haramaya University, P.O. Box 235, Harar, Ethiopia

    Balakrishnan Senthilkumar

  2. Center for Immunology and Microbial Disease, Albany Medical College, Albany, NY, 12208-3479, USA

    Periasamy Sivakumar

  3. Department of Biotechnology, Muthayammal College of Arts and Science, Rasipuram, 637 408, Tamil Nadu, India

    Balakrishnan Senthilkumar, Rajendran Madhanraj, Duraisamy Senbagam & Sivaji Illakia

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  1. Balakrishnan Senthilkumar
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  2. Periasamy Sivakumar
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  3. Rajendran Madhanraj
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  4. Duraisamy Senbagam
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  5. Sivaji Illakia
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Correspondence to Balakrishnan Senthilkumar.

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Senthilkumar, B., Sivakumar, P., Madhanraj, R. et al. A comparative analysis of TLR5 polymorphism and clinical parameters in typhoid patients and asymptomatic typhoid carriers. J Public Health 22, 131–137 (2014). https://doi.org/10.1007/s10389-013-0604-5

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  • DOI: https://doi.org/10.1007/s10389-013-0604-5

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