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Pre-banking microbial contamination of donor conjunctiva and storage medium for penetrating keratoplasty

  • Clinical Investigation
  • Published:
Japanese Journal of Ophthalmology Aims and scope Submit manuscript

Abstract

Purpose

The aims of this study were to investigate the incidence of positive donor tissue cultures before transfer to preservation medium (Optisol™-GS) for penetrating keratoplasty, to verify the efficacy of antibiotics contained in Optisol™-GS by examining the drug susceptibility and to assess the relationship between the results of our microbial assessments as well as donor factors and the incidence of contamination.

Methods

We conducted a retrospective, cross-sectional study using Juntendo Eye Bank records for all corneal transplantations. Two hundred donor conjunctiva harvestings and storage medium (EP-II®) cultures were performed between July 2008 and June 2011. We analyzed the associations between donor factors (age, gender, history of cataract surgery, death-to-preservation interval, cause of death) and contamination rates using multivariate analysis by the generalized estimating equation model.

Results

We obtained positive bacterial cultures from 154 of the 200 eyes (77.0%). The isolated bacteria were indigenous, such as coagulase-negative Staphylococci, Corynebacterium sp., and methicillin-resistant Staphylococcus aureus (MRSA). There was significant resistance to levofloxacin (18 eyes, 9.0%) and gentamicin (12 eyes, 6.0%), and no vancomycin-resistant bacteria were detected. The donor factors did not correlate with the prevalence of bacterial contamination in our criteria.

Conclusions

Pre-banking microbial assessment allows for microbial detection, bacterial susceptibility and resistance testing. This is useful for developing preservation mediums containing effective spectrum antibiotic agents for high quality control of corneal banking.

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Acknowledgements

We thank Juntendo Eye Bank, Japan Organ Transplant Network and Oyama Lions Club for their cornea donations. The authors thank Dr. Alireza Mashaghi, Dr. Jiaxu Hong and Dr. Masahiro Nakamura for critically reading and editing the manuscript, Dr. Ayako Nakamura for her microbiological insight and Peter Mallen for his assistance in creating the elegant figure.

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Authors and Affiliations

  1. Department of Ophthalmology, Juntendo University Faculty of Medicine, Bunkyo-ku, 2-1-1 Hongo, Tokyo, 113-0033, Japan

    Takenori Inomata, Satoru Nakatani, Masahiro Yamaguchi & Akira Murakami

  2. Department of Ophthalmology, Juntendo Tokyo Koto Geriatric Medical Center, Juntendo University School of Medicine, Koto-ku, Tokyo, Japan

    Koichi Ono

  3. Advanced Research Center for Human Sciences, Waseda University, Tokorozawa, Saitama, Japan

    Tsuyoshi Matsuba

  4. Department of Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA, USA

    Tina Shiang

  5. BioMolecular and Cellular Laboratories in Ophthalmology, IRCCS, G.B. Bietti Foundation, Rome, Italy

    Antonio Di Zazzo

  6. Department of Ophthalmology, Juntendo University Urayasu Hospital, Tomioka, Chiba, Japan

    Nobuyuki Ebihara

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  1. Takenori Inomata
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  2. Koichi Ono
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Correspondence to Takenori Inomata.

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Inomata, T., Ono, K., Matsuba, T. et al. Pre-banking microbial contamination of donor conjunctiva and storage medium for penetrating keratoplasty. Jpn J Ophthalmol 61, 369–377 (2017). https://doi.org/10.1007/s10384-017-0521-1

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  • DOI: https://doi.org/10.1007/s10384-017-0521-1

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