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Neuroprotective Effect of the Novel Na+/Ca2+ Channel Blocker NS-7 on Rat Retinal Ganglion Cells

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Abstract

Purpose

To investigate whether NS-7, 4-(4-fluorophenyl)-2-methyl-6-(5-piperidinopentyloxy) pyrimidine hydrochloride, a novel Na+/Ca2+ channel blocker, can protect the rat retina subjected to ischemia–reperfusion insult.

Methods

To evaluate the protective effect of NS-7 against retinal damage, the drug was administered before and after ischemia–reperfusion. Damage to the retina was assessed by measuring the thickness of the inner plexiform layer (IPL) and the outer nuclear layer (ONL) of each eye. In a subsequent experiment, electroretinographic (ERG) evaluation was also used.

Results

In histopathologic evaluation, ischemia–reperfusion injury caused a significant reduction of IPL thickness (measured as the IPL/ONL ratio). In the NS-7-treated group, retinal damage was partially prevented by a concentration of 0.25 mg/kg per day. In the ERG evaluation, ischemia–reperfusion injury caused a reduction of A- and B-wave amplitudes. NS-7 treatment significantly prevented the reduction of the B wave at a concentration of 0.1 or 0.3 mg/kg, while the reduction of the A wave was not significantly affected.

Conclusions

NS-7 has neuroprotective effects against retinal damage resulting from subjection to ischemia. In addition, NS-7 can be used as an agent for treating acute ischemic retinopathy, including diseases associated with very high intraocular pressure, such as acute angle-closure glaucoma. Jpn J Ophthalmol 2005;49:371–376 © Japanese Ophthalmological Society 2005

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Author information

Authors and Affiliations

  1. Department of Ophthalmology, Nippon Medical School, Tokyo, Japan

    Shinichiro Saito

  2. Department of Ophthalmology, University of Tokyo School of Medicine, Tokyo, Japan

    Masaaki Ohashi & Makoto Araie

  3. Sagami Research Laboratory, Wakamoto Pharmaceutical Company, Ltd., Sagami, Japan

    Akira Naito & Yasuhiro Fukaya

  4. Department of Ophthalmology, Teikyo University, Ichihara Hospital, Ichihara, Japan

    Yasuyuki Suzuki

Authors
  1. Shinichiro Saito
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  2. Masaaki Ohashi
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  3. Akira Naito
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  4. Yasuhiro Fukaya
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Correspondence to Shinichiro Saito.

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Saito, S., Ohashi, M., Naito, A. et al. Neuroprotective Effect of the Novel Na+/Ca2+ Channel Blocker NS-7 on Rat Retinal Ganglion Cells. Jpn J Ophthalmol 49, 371–376 (2005). https://doi.org/10.1007/s10384-005-0210-3

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  • DOI: https://doi.org/10.1007/s10384-005-0210-3

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