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Training increases peroxiredoxin 2 contents in the erythrocytes of overweight/obese men suffering from type 2 diabetes

Training erhöht die Peroxiredoxin-2-Gehalte in den Erythrozyten übergewichtiger/adipöser Typ-2-Diabetiker

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Zusammenfassung

Typ-2-Diabetes mellitus (T2DM) ist mit einem erhöhten Aufkommen freier Radikale assoziiert, die eine entscheidende Rolle in der Manifestation des Diabetes und in der Progression diabetischer Komplikationen spielen. Peroxiredoxine sind bedeutsame Komponenten der erythrozytären antioxidativen Abwehr. Daher verglichen wir die Gehalte an Peroxiredoxin-Isoformen (PRDX1-6 immunohistochemische Färbungen) in den Erythrozyten von übergewichtigen/adipösen T2DM-Männern (n = 6) und von BMI-ähnlichen nicht-diabetischen männlichen Kontrollpersonen (n = 6). Nur die erythrozytären Proteine PRDX1 und PRDX2 konnten immunohistochemisch detektiert werden. PRDX1 war signifikant erhöht bei T2DM-Männern im Vergleich zu den Kontrollpersonen (+95,9 %, P ≤ 0,05). Desweiteren haben wir den Einfluss eines 3-monatigen Ausdauer-Trainingsprogrammes (3mal wöchentlich, Fahrradfahren bei 75 % der maximalen Herzfrequenz) auf die Gehalte an PRDX1 und PRDX2 in den Erythrozyten übergewichtiger/adipöser T2DM-Männer (n = 11) untersucht. Das Training erhöhte basales PRDX2 signifikant (+96 %, P ≤ 0,05). Die Hochregulierung des Peroxiredoxin-Systems könnte dabei helfen, freie Radikale in den Erythrozyten von T2DM-Patienten zu puffern.

Summary

Type 2 diabetes mellitus (T2DM) is associated with an increased release of free radicals which play an important role in the manifestation of diabetes and in the progression of diabetic complications. Peroxiredoxins are thought to be essential components of the erythrocyte antioxidative defense. Therefore, we compared peroxiredoxin isoform contents (PRDX1-6 immunohistochemial stainings) in the erythrocytes of overweight/obese T2DM men (n = 6) and of BMI-matched non-diabetic male control subjects (n = 6). Only erythrocyte PRDX1 and PRDX2 proteins were detectable using immunohistochemical methods. PRDX1 was significantly increased in T2DM men relative to control subjects (+95.9%, P ≤ 0.05). Furthermore, we studied the influence of a 3-month endurance training program (3 times a week, cycling at 75% maximal heart rate) on erythrocyte PRDX1 and PRDX2 contents in overweight/obese T2DM men (n = 11). Training significantly increased PRDX2 at rest (+96%, P ≤ 0.05). The up-regulation of the peroxiredoxin system may help counteract free radicals in the erythrocytes of T2DM patients.

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References

  • Wild S, Roglich G, Green A, et al. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care, 27: 1047–1053, 2004

    Article  PubMed  Google Scholar 

  • Dröge W. Free Radicals in the Physiological Control of Cell Function. Physiol Rev, 82: 47–95, 2002

    PubMed  Google Scholar 

  • Valko M, Leibfritz D, Moncol J, et al. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol, 39: 44–84, 2007

    Article  PubMed  CAS  Google Scholar 

  • Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature, 414: 813–820, 2001

    Article  PubMed  CAS  Google Scholar 

  • Kaneto H, Katakami N, Matsuhisa M, et al. Role of reactive oxygen species in the progression of type 2 diabetes and atherosclerosis. Mediators Inflamm, 2010: 453892, 2010

    Article  PubMed  Google Scholar 

  • Li JM, Shah AM. ROS generation by nonphagocytic NADPH oxidase: Potential relevance in diabetic nephropathy. J Am Soc Nephrol, 14: 221–226, 2003

    Article  Google Scholar 

  • Wolff SP, Dean RT. Glucose autooxidation and protein modification. Biochem J, 245: 243–250, 1987

    PubMed  CAS  Google Scholar 

  • Wood ZA, Schröder E, Robin Harris J, et al. Structure, mechanism and regulation of peroxiredoxins. Trends Biochem Sci, 28: 32–40, 2003

    Article  PubMed  CAS  Google Scholar 

  • Minetti M, Agati L, Malorni W. The microenvironment can shift erythrocytes from a friendly to a harmful behavior: pathogenetic implications for vascular diseases. Cardiovasc Res, 75: 21–28, 2007

    Article  PubMed  CAS  Google Scholar 

  • Low FM, Hampton MB, Peskin AV, et al. Peroxiredoxin 2 functions as a noncatalytic scavenger of low-level hydrogen peroxide in the erythrocyte. Blood, 109: 2611–2617, 2007

    Article  PubMed  CAS  Google Scholar 

  • Ji LL. Antioxidants and oxidative stress in exercise. Proc Soc Exp Biol Med, 222: 283–292, 1999

    Article  PubMed  CAS  Google Scholar 

  • Lovlin R, Cottle W, Pyke I, et al. Are indices of free radical damage related to exercise intensity. Eur J Appl Physiol Occup Physiol, 56: 313–316, 1987

    Article  PubMed  CAS  Google Scholar 

  • Miyazaki H, Oh-ishi S, Ookawara T, et al. Strenuous endurance training in humans reduces oxidative stress following exhausting exercise. Eur J Appl Physiol, 84: 1–6, 2001

    Article  PubMed  CAS  Google Scholar 

  • Nikolaidis MG, Kyparos A, Hadziioannou M, et al. Acute exercise markedly increases blood oxidative stress in boys and girls. Appl Physiol Nutr Metab, 32: 197–205, 2007

    Article  PubMed  CAS  Google Scholar 

  • Coskun O, Ocakci A, Bayraktaroglu T, et al. Exercise training prevents and protects streptozotocin-induced oxidative stress and beta-cell damage in rat pancreas. Tohoku J Exp Med, 203: 145–154, 2004

    Article  PubMed  CAS  Google Scholar 

  • Iborra RT, Ribeiro IC, Neves MQ, et al. Aerobic exercise training improves the role of high-density lipoprotein antioxidant and reduces plasma lipid peroxidation in type 2 diabetes mellitus. Scand J Med Sci Sports, 18: 742–750, 2008

    Article  PubMed  CAS  Google Scholar 

  • Roberts CK, Vaziri ND, Barnard RJ. Effect of diet and exercise intervention on blood pressure, insulin, oxidative stress, and nitric oxide availability. Circulation, 106: 2530–2532, 2002

    Article  PubMed  CAS  Google Scholar 

  • Brinkmann C, Chung N, Schmidt U, et al. Training alters the skeletal muscle antioxidative capacity in non-insulin-dependent type 2 diabetic men. Scand J Med Sci Sports 2011 April 8. doi: 10.1111/j.1600-0838.2010.01273.x [Epub ahead of print]

  • Sigal RJ, Kenny GP, Wasserman DH, et al. Physical activity/exercise and type 2 diabetes. Diabetes Care, 27: 2518–2539, 2004

    Article  PubMed  Google Scholar 

  • Fischer UM, Schindler R, Brixius K, et al. Extracorporeal circulation activates endothelial nitric oxide synthase in erythrocytes. Ann Thorac Surg, 84: 2000–2003, 2007

    Article  PubMed  Google Scholar 

  • Suhr F, Porten S, Hertrich T, et al. Intensive exercise induces changes of endothelial nitric oxide synthase pattern in human erythrocytes. Nitric Oxide, 20: 95–103, 2009

    Article  PubMed  CAS  Google Scholar 

  • Low FM, Hampton MB, Winterbourn CC. Peroxiredoxin 2 and peroxide metabolism in the erythrocyte. Antioxid Redox Signal, 10: 1621–1630, 2008

    Article  PubMed  CAS  Google Scholar 

  • Manta B, Hugo M, Ortiz C, et al. The peroxidase and peroxynitrite reductase activity of human erythrocyte peroxiredoxin 2. Arch Biochem Biophys, 484: 146–154, 2009

    Article  PubMed  CAS  Google Scholar 

  • Bhattacharya D, Mukhopadhyay D, Chakrabarti A. Hemoglobin depletion from red blood cell cytosol reveals new proteins in 2-D gel-based proteomics study. Proteomics Clin Appl, 1: 561–564, 2007

    Article  PubMed  CAS  Google Scholar 

  • Goodman SR, Kurdia A, Ammann L, et al. The human red blood cell proteome and interactome. Exp Biol Med (Maywood), 232: 1391–1408, 2007

    Article  CAS  Google Scholar 

  • Kakhniashvili DG, Bulla LA Jr, Goodman SR. The human erythrocyte proteome: analysis by ion trap mass spectrometry. Mol Cell Proteomics, 3: 501–509, 2004

    Article  PubMed  CAS  Google Scholar 

  • Memisogullari R, Taysi S, Bakan E, et al. Antioxidant status and lipid peroxidation in type II diabetes mellitus. Cell Biochem Funct, 21: 291–296, 2003

    Article  PubMed  CAS  Google Scholar 

  • Egler RA, Fernandes E, Rothermund K, et al. Regulation of reactive oxygen species, DNA damage, and c-Myc function by peroxiredoxin 1. Oncogene, 24: 8038–8050, 2005

    Article  PubMed  CAS  Google Scholar 

  • Mueller S, Riedel HD, Stremmel W. Direct evidence for catalase as the predominant H2O2 – removing enzyme in human erythrocytes. Blood, 90: 4973–4978, 1997

    PubMed  CAS  Google Scholar 

  • Johnson RM, Goyette G Jr, Ravindranath Y, et al. Hemoglobin autoxidation and regulation of endogenous H2O2 levels in erythrocytes. Free Radic Biol Med, 39: 1407–1417, 2005

    Article  PubMed  CAS  Google Scholar 

  • Lee TH, Kim SU, Yu SL, et al. Peroxiredoxin II is essential for sustaining life span of erythrocytes in mice. Blood, 101: 5033–5038, 2003

    Article  PubMed  CAS  Google Scholar 

  • Neumann CA, Krause DS, Carman CV, et al. Essential role for the peroxiredoxin Prdx1 in erythrocyte antioxidant defence and tumour suppression. Nature, 424: 561–565, 2003

    Article  PubMed  CAS  Google Scholar 

  • Rabilloud T, Berthier R, Vinçon M, et al. Early events in erythroid differentiation: accumulation of the acidic peroxidoxin (PRP/TSA/NKEF-B). Biochem J, 312: 699–705, 1995

    PubMed  CAS  Google Scholar 

  • Shin YA, Lee JH, Song W, et al. Exercise training improves the antioxidant enzyme activity with no changes of telomere length. Mech Ageing Dev, 129: 254–260, 2008

    Article  PubMed  CAS  Google Scholar 

  • Lau CC, Kuk F. Enough is enough: a primer on power analysis in study designs. The Hearing Journal, 64: 30–39, 2011

    Google Scholar 

  • Ide T, Tsutsui H, Ohashi N, et al. Greater oxidative stress in healthy young men compared with premenopausal women. Arterioscler Thromb Vasc Biol, 22: 438–442, 2002

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany

    David Assadi Moghaddam, Anke Heber, Dario Capin, Thorsten Kreutz, David Opitz, Edward Lenzen, Wilhelm Bloch, Klara Brixius & Christian Brinkmann

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  1. David Assadi Moghaddam
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  2. Anke Heber
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  3. Dario Capin
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  4. Thorsten Kreutz
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  5. David Opitz
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  7. Wilhelm Bloch
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  8. Klara Brixius
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  9. Christian Brinkmann
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Moghaddam, D., Heber, A., Capin, D. et al. Training increases peroxiredoxin 2 contents in the erythrocytes of overweight/obese men suffering from type 2 diabetes. Wien Med Wochenschr 161, 511–518 (2011). https://doi.org/10.1007/s10354-011-0037-0

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  • DOI: https://doi.org/10.1007/s10354-011-0037-0

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