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Exposure to spinosad affects orb-web spider (Agalenatea redii) survival, web construction and prey capture under laboratory conditions

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Abstract

Spinosad is a neurotoxic pesticide, which is currently used in IPM and organic agriculture. It can affect the survival and ecological function of spiders, which are natural enemies of important agricultural pests. In the laboratory, we carried out tests to determine lethal and sublethal effects of spinosad on mortality, web building, and web characteristics of Agalenatea redii. Spinosad has a lethal effect at the normal application rate (NAR, i.e., 96 g ha−1) causing 35 % mortality (vs 0 % for control) after 4 days and 62 % mortality (vs 14 % for control) after 30 days. For the sublethal effects, web building was affected and fewer spiders built webs when exposed to spinosad (10/37 at NAR and 28/39 at half the NAR vs 35/37 for control group). No delay in web building was observed following exposure. Spider webs showed irregularities in the spiral-thread spacing (parallelism) when exposed to higher doses of spinosad (NAR and half of the NAR). Spinosad also affected prey capture: spiders exposed to spinosad (NAR) showed decreased prey capture efficiency (32 vs 73 % for control). These results showed that spinosad affects the spider predatory behavior (agriculture auxiliaries), which could modify their role in pest biocontrol.

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Acknowledgments

The University of Lorraine (Unit of Research in Functions and Animal Product, URAFPA) supported this study. We thank Joel Couturier who helped us rear the spiders in the laboratory and two anonymous reviewers for their helpful comments on an earlier draft of the paper.

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Authors and Affiliations

  1. CNRS, INRA, UR AFPA, Faculty of Sciences and Technologies, University of Lorraine, 54506, Vandoeuvre Les Nancy, France

    Alain Pasquet & Nora Tupinier

  2. UMR 7263 IMBE, UAPV - CNRS - IRD – AMU, Université d’Avignon, 301 rue Baruch de Spinoza, BP21239, 84916, Avignon Cedex 09, France

    Christophe Mazzia

  3. INRA, UR1115 “Plantes et Sytèmes de Culture Horticoles”, Site Agroparc, 84914, Avignon Cedex 09, France

    Yvan Capowiez

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  1. Alain Pasquet
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  2. Nora Tupinier
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  4. Yvan Capowiez
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Correspondence to Alain Pasquet.

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Communicated by J. J. Duan.

Alain Pasquet and Nora Tupinier were first co-authors.

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Pasquet, A., Tupinier, N., Mazzia, C. et al. Exposure to spinosad affects orb-web spider (Agalenatea redii) survival, web construction and prey capture under laboratory conditions. J Pest Sci 89, 507–515 (2016). https://doi.org/10.1007/s10340-015-0691-x

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  • DOI: https://doi.org/10.1007/s10340-015-0691-x

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