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A Liquid Chromatographic Analysis of Deferasirox in Human Breast Milk with Fluorimetric Detection

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Abstract

To determine deferasirox in human breast milk a novel, sensitive high-performance liquid chromatographic method with fluorescence detection has been developed. Precolumn derivatization process is applied to achieve fluorescent analyte using 4-bromomethyl-7-methoxy coumarin (BrMmC) as a reagent and dibenzo-18-crown-6-ether as catalyst. The method depends on reverse phase chromatography. C18 column has been used with 150 × 4.6 mm, 5 μm particle size and the mobile phase consisting of acetonitrile:phosphate buffer (20 mM, pH 3) (70:30, v/v) with a flow rate of 1.0 mL/min. Fluorescence detection at 390 nm is provided with an excitation at 320 nm The linear range is 0.01–1.0 μg/mL. The mean recovery has been calculated as 100.7% with a relative standard deviation (RSD) value less than 2%. The developed method was successfully applied for the determination of deferasirox in human breast milk samples.

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Author notes

  1. Cem Onal

    Present address: Faculty of Pharmacy, Department of Analytical Chemistry, Istanbul Health and Technology University, Istanbul, Turkey

Authors and Affiliations

  1. CinnaGen Pharmaceutical, Atasehir, Istanbul, Turkey

    Cem Onal

  2. Faculty of Pharmacy, Department of Analytical Chemistry, Bezmialem Vakif University, Fatih, Istanbul, Turkey

    Serife Evrim Kepekci Tekkeli

  3. Faculty of Pharmacy, Department of Pharmaceutical Technology, Bezmialem Vakif University, Fatih, Istanbul, Turkey

    Ali Asram Sagiroglu

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  1. Cem Onal
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  2. Serife Evrim Kepekci Tekkeli
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  3. Ali Asram Sagiroglu
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Onal, C., Tekkeli, S.E.K. & Sagiroglu, A.A. A Liquid Chromatographic Analysis of Deferasirox in Human Breast Milk with Fluorimetric Detection. Chromatographia 83, 1329–1333 (2020). https://doi.org/10.1007/s10337-020-03953-5

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