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Determination of 17 Phthalate Esters in Infant Milk Powder and Dairy Products by GC–MS with 16 Internal Standards

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Abstract

In this study, a simple, rapid and accurate quantitative method for the determination of phthalate esters in infant milk powder and dairy products by GC–MS using 16 internal standards has been developed. Samples were extracted with acetonitrile and purified using PAE solid-phase extraction (SPE), the eluant was then dried with N2 flow at 45 °C and diluted with n-hexane for testing by GC–MS. The standard calibration curves were linear for all matrixes analysed, and the correlation coefficients ranged from 0.9955 to 0.9999. The LODs and LOQs were in the ranges of 1.0–100.0 µg kg−1 and 3.0–300.0 µg kg−1, respectively. The accuracy of this method was evaluated by measuring the recovery from spiked samples. The recoveries of all phthalates from samples spiked at three different concentrations (100.0, 500.0, and 1000.0 µg kg−1) were determined. The recovery ranges in low, intermediate and high spiked levels were 73.8–114.5 %, 76.1–102.2 % and 81.1–113.5 %, respectively, and the relative standard deviation (RSD) range of recovery results (n = 6) was 0.5–19.1 %, 0.3–6.4 % and 0.2–19.2 % at low, intermediate and high spiked levels. Furthermore, the differences in recovery and RSD were discussed when different quantitative methods were used. In addition, the matrix effect was discussed in the article.

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Acknowledgments

This work was supported by Hangzhou Health Science and technology project (2010B042).

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Authors and Affiliations

  1. Hangzhou Center for Disease Control and Prevention, Hangzhou, 310021, People’s Republic of China

    Ren Ren, Quan Jin, Hua-li He, Tian-bin Bian, Shu-ting Wang & Ji-cai Fan

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  1. Ren Ren
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  2. Quan Jin
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  3. Hua-li He
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  4. Tian-bin Bian
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  5. Shu-ting Wang
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  6. Ji-cai Fan
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Correspondence to Ji-cai Fan.

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Ren, R., Jin, Q., He, Hl. et al. Determination of 17 Phthalate Esters in Infant Milk Powder and Dairy Products by GC–MS with 16 Internal Standards. Chromatographia 79, 903–910 (2016). https://doi.org/10.1007/s10337-016-3102-4

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  • DOI: https://doi.org/10.1007/s10337-016-3102-4

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