Skip to main content
SpringerLink
Log in

Stability-Indicating HPLC and RP-TLC Determination of Cefpirome Sulfate with Kinetic Study

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

An Erratum to this article was published on 28 January 2014

Abstract

Two sensitive and selective stability-indicating methods were developed for the determination of the antibiotic cefpirome sulfate in bulk powder, pharmaceutical formulation and in presence of its acid, alkaline, photo- and oxidative degradation products. Method A was based on HPLC separation of cefpirome sulfate in the presence of its degradation products on a reversed phase column C18, 250 × 4.6 mm, 5-μm particle size and mobile phase consisting of 0.1 M disodium hydrogen phosphate dihydrate pH 3.9 adjusted with phosphoric acid–acetonitrile (85:15, v/v). Quantitation was achieved with UV detection at 270 nm. The linear calibration curve was in the range 5.0–50.0 μg mL−1. Method B was based on reversed phase TLC separation of the cited drug in the presence of its degradation products followed by densitometric measurement of the intact drug at 270 nm. The separation was carried out using disodium hydrogen phosphate dihydrate 2.0 g %w/v, at pH 3.5 adjusted with phosphoric acid–acetone (15:10, v/v) as a developing system. The calibration curve was in the range of 1.0–10.0 μg/spot. The HPLC method was used to study the kinetic of cefpirome sulfate acid degradation. The results obtained were statistically analyzed and compared with those obtained by applying the official Japanese method.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. The Japanese Pharmacopoeia (2006) 15th edition English Version, Tokyo

  2. Sweetman S (2011) Martindale “The extra pharmacopoeia”, The Complete Drug Reference 37th edition, The Pharmaceutical Press, London

  3. Turley C, Kearns G, Jacobos R (1988) Micro-analytical HPLC assay for cefpirome (HR810) in serum. Antimicrob Agen Chemoth 32(10):1481–1483

    Article  CAS  Google Scholar 

  4. Sugioka T, Asano T, Chikaraishi Y, Suzuki E, Sano A, Kuriki T, Shirotsuka M, Satio K (1990) Stability and degradation pattern of cefpirome (HR 810) in aqueous solution. Chem Pharm Bull 38(7):1998–2002

    Article  CAS  Google Scholar 

  5. Nahata M (1991) Determination of cefpirome in human plasma by HPLC. J Liq Chromatogr 14(1):193–200

    Article  CAS  Google Scholar 

  6. Tabata S, Koike Y, Sato Y, Kobayashi I, Hirayama M, Hasegawa Y (1991) Determination method and stability of cefpirome sulfate in biological samples. Chemoth Tokyo 39(1):92–99

    CAS  Google Scholar 

  7. Kearns G, Johnson V, Hendry I, Wells T (1992) Microanalytical HPLC assay for cefpirome in human milk and urine. J Chromatogr 574(2):356–360

    Article  CAS  Google Scholar 

  8. Pehourcq F, Jarry C (1998) Determination of third-generation cephalosporins by high performance liquid chromatography in connection with pharmacokinetic studies. J Chromatogr A 812(1–2):159–178

    Article  CAS  Google Scholar 

  9. Breilh D, Lavallee C, Fratta A, Ducint D, Cony-Maklouf P, Saux M (1999) Determination of cefepime and cefpirome in human serum by high-performance liquid chromatography using an ultrafiltration for antibiotics serum extraction. J Chromato B., Biomed Sci Appl 734(1):121–127

    Article  CAS  Google Scholar 

  10. Cherti N, Kinowski J, Lerfant J, Bressolle F (2001) High-performance liquid-chromatographic determination of cefepime in human plasma and in urine and dialysis fluid using a column-switching technique. J Chromato B Biomed Sci Appl 754(2):377–386

    Article  CAS  Google Scholar 

  11. Arayne M, Sultana N, Nawaz M (2008) Simultaneous quantification of cefpirome and cetirizine or levocetirizine in pharmaceutical formulations and human plasma by RP-HPLC. J Anal Chem 63(9):881–887

    Article  CAS  Google Scholar 

  12. Rao K, Kumar K, Joydeep D (2011) New stability indicating RP-HPLC method for the estimation of cefpirome sulphate in bulk and pharmaceutical dosage forms. Sci Pharm 79:899–907

    Article  CAS  Google Scholar 

  13. Dhanesar S (1999) Quantitation of antibiotics by densitometry on a hydrocarbon-impregnated silica gel HPTLC plate, part III: quantitation and evaluation of cephalosporins. J Plan Chromato Mod TLC 12(2):114–119

    CAS  Google Scholar 

  14. Mayer B, Hollenstein U, Brunner M, Eichler H, Mueller M (2000) Micellar electrokinetic chromatography for the analysis of cefpirome in microdialysis and plasma samples obtained in vivo from human volunteers. Electrophoresis 21(8):1558–1564

    Article  CAS  Google Scholar 

  15. Prakash K, Rao J, Raju N, Himabindu V (2008) Spectrophotometric estimation of cefpirome sulfate in pharmaceutical formulations. Asian J Chem 20(4):2587–2590

    CAS  Google Scholar 

  16. Evagelou V, Tsantili-Kakoulidou A, Koupparis M (2003) Determination of the dissociation constants of the cephalosporins, cefepime and cefpirome using UV spectrometry and pH potentiometry. J Pharm Biomed Anal 31(6):1119–1128

    Article  CAS  Google Scholar 

  17. National Committee for Clinical Laboratory Standards (1993) Performance standards for antimicrobial disc susceptibility tests. Approved standard NCCLS publication M2-A5. Villanova, USA

    Google Scholar 

  18. International Conference of Harmonization (ICH) of Technical Requirements for the Registration of Pharmaceuticals for Human Use, Validation of Analytical Procedures: Definitions and Terminology, Stability Testing of New Drug Substances and Products Q1A (R2) (2003) Geneva

  19. Sainsbury M (2001) Heterocyclic chemistry. The Royal Society of Chemistry, UK, pp 18–22

    Book  Google Scholar 

  20. Begtrup M, Hansen L (1992) New methods for the introduction of substituents into thiazole. Acta Chem Scand 46:372–383

    Article  CAS  Google Scholar 

  21. The United States Pharmacopeia and National Formulary. USP 34-NF 29 (2011) US pharmacopeial convention. Rockville, USA

    Google Scholar 

  22. Florence A, Attwood D (1998) Physical principles of pharmacy, 2nd edn. Macmillan, London

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., 11562, Cairo, Egypt

    Mohamed R. Elghobashy & Samah S. Abbas

  2. National Organization for Drug Control and Research (NODCAR), 51 Wezaret El-Zeraa St., Dokki, Cairo, Egypt

    Lories I. Bebawy & Rafeek F. Shokry

Authors
  1. Mohamed R. Elghobashy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lories I. Bebawy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Samah S. Abbas
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rafeek F. Shokry
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mohamed R. Elghobashy.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Elghobashy, M.R., Bebawy, L.I., Abbas, S.S. et al. Stability-Indicating HPLC and RP-TLC Determination of Cefpirome Sulfate with Kinetic Study. Chromatographia 76, 1141–1151 (2013). https://doi.org/10.1007/s10337-013-2516-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10337-013-2516-5

Keywords

Search

Navigation