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A Sensitive LC–MS–MS Method for Simultaneous Quantification of Two Structural Isomers, Hyperoside and Isoquercitrin: Application to Pharmacokinetic Studies

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Abstract

We report the development and validation of a rapid, specific, and sensitive liquid chromatographic–tandem mass spectrometric (LC–MS–MS) method for analysis and pharmacokinetic study, in rats, of hyperoside and isoquercitrin, two bioactive structural isomers present in the leaves of Apocynum venetum L. After simple deproteinization by addition of acetonitrile, the analytes were separated on a C18 column. Detection was by tandem mass spectrometry in multiple reaction monitoring mode. The method was linear over the concentration range 3.9–195 ng mL−1 for both hyperoside and isoquercitrin. Intra-day and inter-day precision for both hyperoside and isoquercitrin were <13.1%, and relative errors were all within 7.1% at all QC levels. The method was used to study the pharmacokinetic performance of the compounds after oral administration of an extract of Apocynum venetum L. leaves to rats.

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Authors and Affiliations

  1. Department of Pharmaceutical Analysis, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China

    Chunling Zhou, Xiaomian Zhou, Xiaohui Chen & Kaishun Bi

  2. Liaoning Provincial Institute for Control of Food and Drugs, 46 Guihe Street, 110023, Shenyang, China

    Chunling Zhou, Yang Liu, Guanghui Gao, Lingling Sun & Xinguo Ba

  3. The National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine, 56 Yangming Road, 330006, Nanchang, China

    Dan Su

  4. Jiangxi University of Chinese Traditional Medicine, 56 Yangming Road, 330006, Nanchang, China

    Dan Su

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  1. Chunling Zhou
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  2. Yang Liu
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  3. Dan Su
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  4. Guanghui Gao
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  5. Xiaomian Zhou
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  8. Xiaohui Chen
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  9. Kaishun Bi
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Correspondence to Kaishun Bi.

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Zhou, C., Liu, Y., Su, D. et al. A Sensitive LC–MS–MS Method for Simultaneous Quantification of Two Structural Isomers, Hyperoside and Isoquercitrin: Application to Pharmacokinetic Studies. Chromatographia 73, 353–359 (2011). https://doi.org/10.1007/s10337-010-1879-0

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  • DOI: https://doi.org/10.1007/s10337-010-1879-0

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