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Rapid Simultaneous Determination of Eight Benzimidazoles in Animal Feed by LC–MS–MS

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Abstract

A rapid, sensitive and reliable LC–MS–MS method for the determination of eight benzimidazoles in animal feed was developed and validated. Samples were extracted with acidic acetonitrile. The extract was diluted with 0.1% formic acid in water, and analyzed by LC–MS–MS on a Waters XBridge™ C18 column with acetonitrile/0.1% formic acid in water as mobile phase. The samples were quantified with the matrix standard calibration curve method. Good linearity was obtained for eight benzimidazoles at a concentration of 0.005–2.5 μg mL−1 with a linear relative coefficient more than 0.990. Recoveries of 84.0–104.0% with CVs of 2.50–7.50% were obtained. Limit of detection was 2.1–63.0 μg kg−1. The method demonstrated to be suitable for the determination of eight benzimidazoles in animal feed samples.

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Authors and Affiliations

  1. College of Agriculture, Hebei University of Engineering, Handan, 056038, People’s Republic of China

    Cun Li

  2. Ningbo Agriculture Products Quality Detector Center, Ningbo, 315040, People’s Republic of China

    Yin-Liang Wu, Ting Yang & Yan Zhang

Authors
  1. Cun Li
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  2. Yin-Liang Wu
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  3. Ting Yang
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  4. Yan Zhang
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Correspondence to Yin-Liang Wu.

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Li, C., Wu, YL., Yang, T. et al. Rapid Simultaneous Determination of Eight Benzimidazoles in Animal Feed by LC–MS–MS. Chromatographia 73, 59–65 (2011). https://doi.org/10.1007/s10337-010-1838-9

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  • DOI: https://doi.org/10.1007/s10337-010-1838-9

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