Abstract
Objective
The aim of this study was to use lung cancer targeting binding polypeptide ZS-9 to screen cDNA library of human lung cancer and obtain ZS-9 specific ligand to confirm tumor marker of non small-cell lung cancer.
Methods
Artificially synthesize biotin labeled peptide ZS-9, anchored ZS-9 in the enzyme label plate coupled by avidin, used ZS-9 as probe to screen cDNA library of human lung cancer, after screening, obtained bacteriophage clone specifically binding with anchored polypeptide ZS-9. Extracted plasmid of bacteriophage and performed sequencing after amplified by PCR.
Results
It was demonstrated by bioinformatic analysis on the sequence of ligand binded by lung cancer specific peptide ZS-9 that the ligand was the cytoskeletal protein periplakin on the surface of lung cancer cells, suggesting that periplakin might be a new marker for non-small-cell lung cancer in lung cancer.
Conclusion
Use specific lung cancer binding peptide to screen new tumor marker periplakin in lung cancer and further studies on its biologic functions in genesis and development of lung cancer are still needed.
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Supported by grants from the Science and Technology Planning Project of Guangdong Province (No. 2010B031600066; No. 2010B031500034; No. 2008B030303008); the Key Scientific Subject Foundation of Ministry of Education of China (No. 208105); and the National Science and Technology Major Projects for New Drugs (No. 2011zx09102-001-31).
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Dai, S., Li, W., Kong, M. et al. Screening study on new tumor marker periplakin for lung cancer. Chin. -Ger. J. Clin. Oncol. 12, 305–309 (2013). https://doi.org/10.1007/s10330-013-1178-y
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DOI: https://doi.org/10.1007/s10330-013-1178-y