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Colletotrichum carthami comb. nov., an anthracnose pathogen of safflower, garland chrysanthemum and pot marigold, revived by molecular phylogeny with authentic herbarium specimens

  • Fungal Diseases
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Abstract

Authentic herbarium specimens of Gloeosporium carthami and G. chrysanthemi as well as live Colletotrichum strains from Carthamus tinctorius, Chrysanthemum coronarium var. spatiosum, and Calendula officinalis were judged to be conspecific based on morphological and molecular phylogenetic analyses. Representative strains from three asteraceous plants were demonstrated by inoculation tests to possess specific pathogenicity to the host plants. We propose a new combination, Colletotrichum carthami, for the anthracnose pathogen of the asteraceous plants, designating our specimen of G. carthami as its epitype. We characterize the species, which differs from the closely related Colletotrichum simmondsii in its pathogenicity and molecular characteristics.

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Acknowledgments

This work was sponsored by research grants from the NIAS Genebank Project in 2003 and Research and Development Projects for Application in Promoting New Policy of Agriculture, Forestry and Fisheries, Japan. We are grateful to Professor Dr. Hideki Takahashi, Hokkaido University Museum, for loaning herbarium specimens of G. carthami and G. chrysanthemi; Dr. Toshihiro Kajiwara for providing valuable suggestions and encouragement; Mr. Hiroei Kanno, Miyagi Prefectural Government, for providing the fungal strain from garland chrysanthemum, Dr. Urlike Damm, Centraalbureau voor Schimmelcultures, for sharing information on the phylogenetic placement of C. carthami; Dr. Kei Sugawara, Yamagata Prefectural Agricultural Technique Improvement Research Office, for the gift of safflower seeds; Dr. Tomiichi Takeda, Yamagata General Agricultural Research Center, for help with the collection of fungal materials; Ms. Kayoko Otsubo for technical support; Ms. Miki Kato, Mr. Haruhiko Kagawa, Mr. Yoshiyuki Ebihara, and Mr. Yoshihiko Kanegae of Chiba Prefectural Agriculture and Forestry Research Center and Ms. Silvia M. Kato of Assoiacao dos Floricultores da Regiao Dutra, Brasil, for assistance in morphological observation and inoculations.

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Authors and Affiliations

  1. Southern Prefectural Horticulture Institute, Chiba Prefectural Agriculture and Forestry Research Center, Yamamoto, Tateyama, Chiba, 294-0014, Japan

    Seiji Uematsu

  2. River Basin Research Center, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Koji Kageyama

  3. Horticultural Research Institute, Toyama Prefectural Agricultural, Forestry & Fisheries Research Center, 288 Goromaru, Tonami, Toyama, 939-1327, Japan

    Jouji Moriwaki

  4. National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602, Japan

    Toyozo Sato

Authors
  1. Seiji Uematsu
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  2. Koji Kageyama
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  3. Jouji Moriwaki
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  4. Toyozo Sato
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Corresponding author

Correspondence to Toyozo Sato.

Additional information

The nucleotide sequence data reported here are available in the DDBJ/EMBL/GenBank databases under the accession numbers AB688776–AB688815, AB696992, AB696993, AB696998 and AB696999.

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Uematsu, S., Kageyama, K., Moriwaki, J. et al. Colletotrichum carthami comb. nov., an anthracnose pathogen of safflower, garland chrysanthemum and pot marigold, revived by molecular phylogeny with authentic herbarium specimens. J Gen Plant Pathol 78, 316–330 (2012). https://doi.org/10.1007/s10327-012-0397-3

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