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White clover mosaic virus-induced gene silencing in pea

  • Viral and Viroid Diseases
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

Double-stranded RNAs formed in secondary structures and replicative intermediates of viral genomes are thought to strongly elicit RNA silencing. This phenomenon is known as virus-induced gene silencing (VIGS). VIGS is a powerful tool for modifying gene expression in host plants. We constructed a virus vector based on White clover mosaic virus (WClMV) and demonstrated VIGS of phytoene desaturase (PDS) in pea. Photobleaching of tissues, caused by VIGS of PDS, was observed in restricted areas of upper leaves and stems. We confirmed that the PDS mRNA and subgenomic RNAs of WClMV were reduced in the photobleached tissues.

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References

  • Beck DL, Forster RLS, Bevan MW, Boxen KA, Lowe SC (1990) Infectious transcripts and nucleotide sequence of cloned cDNA of the potexvirus white clover mosaic virus. Virology 177:152–158

    Article  PubMed  CAS  Google Scholar 

  • Becker A, Lange M (2010) VIGS—genomics goes functional. Trends Plant Sci 15:1–4

    Article  PubMed  CAS  Google Scholar 

  • Chapman S, Kavanagh T, Baulcombe D (1992) Potato virus X as a vector for gene-expression in plants. Plant J 2:549–557

    PubMed  CAS  Google Scholar 

  • Constantin GD, Krath BN, MacFarlane SA, Nicolaisen M, Johansen IE, Lund OS (2004) Virus-induced gene silencing as a tool for functional genomics in a legume species. Plant J 40:622–631

    Article  PubMed  CAS  Google Scholar 

  • Demmig-Adams B, Adams WW (1996) The role of xanthophyll cycle carotenoids in the protection of photosynthesis. Trends Plant Sci 1:21–26

    Article  Google Scholar 

  • Forster RLS, Bevan MW, Harbison SA, Gardner RC (1988) The complete nucleotide sequence of the potexvirus white clover mosaic virus. Nucleic Acids Res 16:291–303

    Article  PubMed  CAS  Google Scholar 

  • Hammond J, Lim HS, Vaira AM, Domier LL, Lee SC, Kim HG (2010) Efficiency of VIGS and gene expression in a novel bipartite potexvirus vector delivery system as a function of strength of TGB1 silencing suppression. Virology 402:149–163

    Article  PubMed  Google Scholar 

  • Holzberg S, Brosio P, Gross C, Pogue GP (2002) Barley stripe mosaic virus-induced gene silencing in a monocot plant. Plant J 30:315–327

    Article  PubMed  CAS  Google Scholar 

  • ICTVdB Management (2006) 00.056.0.01.021. White clover mosaic virus. In: Büchen-Osmond C (ed) ICTVdB—the universal virus database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

  • Igarashi A, Yamagata K, Sugai T, Takahashi Y, Sugawara E, Tamura A, Yaegashi H, Yamagishi N, Takahashi T, Isogai M, Takahashi H, Yoshikawa N (2009) Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes. Virology 386:407–416

    Article  PubMed  CAS  Google Scholar 

  • Iizuka N, Iida W (1965) Studies on the white clover mosaic virus. Ann Phytopathol Soc Japan 30:46–53

    Article  Google Scholar 

  • Kumagai MH, Donson J, Della-Cioppa G, Harvey D, Hanley K, Grill LK (1995) Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA. Proc Natl Acad Sci USA 92:1679–1683

    Article  PubMed  CAS  Google Scholar 

  • Lacomme C, Hrubikova K, Hein I (2003) Enhancement of virus-induced gene silencing through viral-based production of inverted-repeats. Plant J 34:543–553

    Article  PubMed  CAS  Google Scholar 

  • Lough TJ, Netzler NE, Emerson SJ, Sutherland P, Carr F, Beck DL, Lucas WJ, Forster RL (2000) Cell-to-cell movement of potexviruses: evidence for a ribonucleoprotein complex involving the coat protein and first triple gene block protein. Mol Plant Microbe Interact 13:962–974

    Article  PubMed  CAS  Google Scholar 

  • Lu R, Malcuit I, Moffett P, Ruiz MT, Peart J, Wu AJ, Rathjen JP, Bendahmane A, Day L, Baulcombe DC (2003) High throughput virus-induced gene silencing implicates heat shock protein 90 in plant disease resistance. EMBO J 22:5690–5699

    Article  PubMed  CAS  Google Scholar 

  • Matsumoto T, Ookawa T, Hashegawa S, Inoue M (1989) Occurrence of viral disease caused by white clover mosaic virus in pea (abstract in Japanese). Ann Phytopathol Soc Japan 55:90

    Google Scholar 

  • Nakabayashi H, Yamaji Y, Kagiwada S, Ugaki M, Namba S (2002) The complete nucleotide sequence of a Japanese isolate of White clover mosaic virus strain RC. J Gen Plant Pathol 68:173–176

    Article  CAS  Google Scholar 

  • Ratcliff FG, MacFarlane SA, Baulcombe DC (1999) Gene silencing without DNA: RNA-mediated cross-protection between viruses. Plant Cell 11:1207–1216

    Article  PubMed  CAS  Google Scholar 

  • Ratcliff F, Martin-Hernandez AM, Baulcombe DC (2001) Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant J 25:237–245

    Article  PubMed  CAS  Google Scholar 

  • Robertson D (2004) VIGS vectors for gene silencing: many targets, many tools. Annu Rev Plant Biol 55:495–519

    Article  PubMed  CAS  Google Scholar 

  • Skryabin KG, Morozov SY, Kraev AS, Rozanov MN, Chernov BK, Lukasheva LI, Atabekov JG (1988) Conserved and variable elements in RNA genomes of potexviruses. FEBS Lett 240:33–40

    Article  PubMed  CAS  Google Scholar 

  • Tsuchizaki T, Goto T, Fujisawa I, Yoshida K (1981) Virus disease occurring on legumes and vegetables in Hokkaido. Res Bull Hokkaido Natl Agric Exp Stn 131:71–93

    Google Scholar 

  • Turnage MA, Muangsan N, Peele CG, Robertson D (2002) Geminivirus-based vectors for gene silencing in Arabidopsis. Plant J 30:107–114

    Article  PubMed  CAS  Google Scholar 

  • van Wezel R, Hong Y (2004) Virus survival of RNA silencing without deploying protein-mediated suppression in Nicotiana benthamiana. FEBS Lett 562:65–70

    Article  PubMed  Google Scholar 

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Acknowledgments

We thank Prof. Shigetou Namba (University of Tokyo, Tokyo, Japan) for WClMV-RC. This work was partly supported by JSPS/MEXT KAKENHI (20688002, 18108001) to K.S.N. and I.U.

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Authors and Affiliations

  1. Pathogen-Plant Interactions Group, Plant Breeding Science, Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan

    Yukari Ido, Kenji S. Nakahara & Ichiro Uyeda

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  1. Yukari Ido
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  2. Kenji S. Nakahara
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  3. Ichiro Uyeda
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Correspondence to Kenji S. Nakahara.

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Ido, Y., Nakahara, K.S. & Uyeda, I. White clover mosaic virus-induced gene silencing in pea. J Gen Plant Pathol 78, 127–132 (2012). https://doi.org/10.1007/s10327-012-0360-3

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  • DOI: https://doi.org/10.1007/s10327-012-0360-3

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