Abstract
Talaromyces sp. isolate KNB-422, isolated from a rice seedling, is a biofungicidal agent effective against several seedborne pathogens of rice including Gibberella fujikuroi, which causes Bakanae disease. Because the fungal mode of action (MOA) has not yet been clarified, we used the fluorescent protein markers GFP and RFP to visualize cell–cell interactions between the biocontrol agent and the pathogen G. fujikuroi. In slide culture, the hyphal cell wall of G. fujikuroi collapsed, and fluorescence of its cytoplasm disappeared 3 days after contact with hyphae of Talaromyces sp. On inoculated rice plants, both fungi occupied the same regions of coleoptiles and roots, where the biocontrol effect of Talaromyces sp. must be exerted. Our observations suggest that the MOA of Talaromyces sp. is mycoparasitic.
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Acknowledgments
We thank Dr. Olen Yoder (Celgene Co., San Diego, CA, USA) for helpful suggestions and discussions when he was a visiting professor at TUAT. This research was partly supported by the grant ‘Development of mitigation and adaptation techniques to global warming in the sectors of agriculture, forestry, and fisheries’ for TA from Ministry of Agriculture Forestry, and Fisheries of Japan.
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10327_2011_343_MOESM1_ESM.ppt
Supplemental Figure Structures of plasmids used in this study. a pMK412 carries egfp driven by the translation elongation factor (TEF) promoter from Aureobasidium pullulans and the glucoamylase (gla) terminator from Aspergillus awamori and a hygromycin B phosphotransferase gene (hph) driven by the trifunctional polypeptide (trpC) promoter and terminator from Aspergillus nidulans. b pDsRed2 (Takara Bio) carries dsred2. c pAK2-HYG, constructed from pMK412 and pDsRed2 in this study, carries dsred2 driven by PTEF and Tgla, and the hph cassette. Black arrows on a and b approximate positions of primers used to construct pAK2-HYG (PPT 252 kb)
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Kato, A., Miyake, T., Nishigata, K. et al. Use of fluorescent proteins to visualize interactions between the Bakanae disease pathogen Gibberella fujikuroi and the biocontrol agent Talaromyces sp. KNB-422. J Gen Plant Pathol 78, 54–61 (2012). https://doi.org/10.1007/s10327-011-0343-9
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DOI: https://doi.org/10.1007/s10327-011-0343-9