Abstract
Potato dextrose broth (PDB), one of the most popular culture media for fungi, can be made in the laboratory from potato extract and glucose (handmade PDB) and also bought as powder (commercial PDB). We compared growth of Fusarium oxysporum in handmade and commercial PDBs. For seven strains tested, bud cell formation and fungal biomass were higher in handmade PDB than in commercial PDB. Gel filtration analyses revealed that handmade PDB contained carbohydrates with molecular weights >20 MDa, while most carbohydrates in commercial PDB were <45 kDa. Freshly prepared, raw potato starch had a molecular weight >20 MDa and enhanced bud cell formation by five of seven strains when added to commercial PDB. The addition of glucose to commercial PDB promoted biomass production but not bud cell formation. Furthermore, in a semisynthetic medium containing raw potato starch, bud cell formation was enhanced for all strains tested. These results indicate that the high molecular carbohydrates present in raw potatoes enhanced bud cell formation by Fusarium oxysporum.
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Acknowledgments
We thank H. Ogiso and M. Fujinaga in Nagano Vegetable and Ornamental Crops Experiment Station and Y. Amemiya in Chiba University for providing Fusarium oxysporum strains. We also thank K. Takano in Tokyo University of Agriculture for his critical advice about carbohydrates. This study was supported by Academic Frontier Research Project in Tokyo University of Agriculture.
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Yokota, K., Teraoka, T., Tsujii, Y. et al. Effect of high molecular weight carbohydrates on bud cell formation by Fusarium oxysporum in potato dextrose broth. J Gen Plant Pathol 76, 219–224 (2010). https://doi.org/10.1007/s10327-010-0238-1
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DOI: https://doi.org/10.1007/s10327-010-0238-1