Abstract
To identify phytoalexins of adzuki bean elicited in response to attempted infection of Phytophthora vignae f. sp. adzukicola, we isolated compounds from adzuki bean and evaluated their antifungal activity. Seven flavonoids (daidzein, genistein, 2′-hydroxygenistein, coumestrol, dalbergioidin, kievitone, and phaseol) were identified from epicotyls wound-inoculated with a mycelial suspension of an avirulent race of P. vignae f. sp. adzukicola. Of those compounds, kievitone and dalbergioidin accumulated to higher levels in incompatible interactions compared to compatible interactions 48 h after inoculation. Kievitone strongly inhibited the germination of encysted zoospores, and dalbergioidin were slightly suppressive. From these results, we concluded that kievitone and dalbergioidin are phytoalexins in adzuki bean.
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Acknowledgments
We are grateful to Dr. Eri Fukushi and Mr. Kenji Watanabe (GC-MS & MNR Laboratory, Graduate School of Agriculture, Hokkaido University) for MS measurements. This study was supported by the Japan Beans Fund Association.
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Harada, G., Kondo, N. Induction of phytoalexins in adzuki bean after inoculation with Phytophthora vignae f. sp. adzukicola . J Gen Plant Pathol 75, 432–436 (2009). https://doi.org/10.1007/s10327-009-0203-z
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DOI: https://doi.org/10.1007/s10327-009-0203-z