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Taxonomic characterization of Pyricularia isolates from green foxtail and giant foxtail, wild foxtails in Japan

  • Fungal Diseases
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Abstract

Twenty-eight Pyricularia isolates from two wild foxtails—green foxtail (Setaria viridis) and giant foxtail (S. faberii)—in Japan were taxonomically characterized by DNA analyses, mating tests, and pathogenicity assays. Although most of the isolates failed to produce perithecia in mating tests with Magnaporthe oryzae, a diagnostic polymerase chain reaction-restriction fragment length polymorphism phenotype of M. oryzae was detected in the beta-tubulin genomic region in all isolates. The pathogenicity assays revealed that host ranges of the isolates were similar to those of isolates from foxtail millet (S. italica), which were exclusively pathogenic on foxtail millet. In addition to the 28 isolates from wild foxtails, 22 Pyricularia isolates from 11 other grasses were analyzed by RFLP using single-copy sequences as probes. In a dendrogram constructed from the RFLP data, isolates that were previously identified as M. oryzae formed a single cluster. All the wild foxtail isolates formed a subcluster with foxtail millet isolates within the M. oryzae cluster. From these results, we conclude that Pyricularia isolates from the wild foxtails are closely related to isolates from foxtail millet and should be classified into the Setaria pathotype of M. oryzae.

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Acknowledgments

We are grateful to Dr. M. Kawase, Genebank, National Institute of Agrobiological Sciences (NIAS), for providing the seeds of the foxtail millet landraces and Dr. M. L. Farman, Department of Plant Pathology, Kentucky University, for providing the plasmid clones used as probes for the scRFLP analysis. Special thanks are due to Dr. H. Yaegashi, former professor at Saga University, for providing valuable suggestions.

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Authors and Affiliations

  1. Laboratory of Plant Pathology, Faculty of Agriculture, Saga University, Honjo1, Saga, 840-8502, Japan

    Akiko Yamagashira, Chihiro Iwai, Masakazu Misaka & Motoaki Kusaba

  2. Department of Medical Genetics, Majors of Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan

    Kenji Hirata

  3. National Agricultural Research Center, Rice Disease Resistance Research Team, Tsukuba, Ibaraki, Japan

    Yoshikatsu Fujita

  4. Laboratory of Plant Pathology, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan

    Yukio Tosa

Authors
  1. Akiko Yamagashira
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  2. Chihiro Iwai
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  3. Masakazu Misaka
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  4. Kenji Hirata
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  5. Yoshikatsu Fujita
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  6. Yukio Tosa
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  7. Motoaki Kusaba
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Correspondence to Motoaki Kusaba.

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Yamagashira, A., Iwai, C., Misaka, M. et al. Taxonomic characterization of Pyricularia isolates from green foxtail and giant foxtail, wild foxtails in Japan. J Gen Plant Pathol 74, 230–241 (2008). https://doi.org/10.1007/s10327-008-0087-3

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  • DOI: https://doi.org/10.1007/s10327-008-0087-3

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