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Identification of cachexia-inducible Hop stunt viroid variants in citrus orchards in Japan using biological indexing and improved reverse transcription polymerase chain reaction

  • VIRAL AND VIROID DISEASES
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Abstract

The distribution of Hop stunt viroid (HSVd) variants related to cachexia disease in Japan was investigated using reverse transcription polymerase chain reaction (RT-PCR) to specifically detect the five nucleotide differences in the “cachexia motif.” With RT-PCR, HSVd variants having the cachexia motif were detected in samples from 10 of 43 citrus trees in experimental orchards and from only 3 of 530 in commercial orchards. Although the trees were symptomless cachexia carriers, biological indexing using “Parson's Special” mandarin (Citrus reticulata Blanco) verified the presence of the cachexia agent. RT-PCR with the primer pair CCV-M1 and CCV-P1 will be useful in preventing the spread of cachexia HSVd variants.

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Correspondence to Takao Ito.

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Contribution no. 1408, of the National Institute of Fruit Tree Science, NARO. The nucleotide sequences for the Hop stunt viroid (HSVd) variants from sample EX with four nucleotide differences and KS14 with five nucleotide differences discriminating cachexia from noncachexia sequences have been deposited in GenBank under accession nos. AB211242 and AB211243.

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Ito, T., Furuta, T., Ito, T. et al. Identification of cachexia-inducible Hop stunt viroid variants in citrus orchards in Japan using biological indexing and improved reverse transcription polymerase chain reaction. J Gen Plant Pathol 72, 378–382 (2006). https://doi.org/10.1007/s10327-006-0303-y

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  • DOI: https://doi.org/10.1007/s10327-006-0303-y

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