Abstract
Two primer sets were designed based on the sequence of polymorphic bands that were derived from repetitive sequence-based polymerase chain reaction (rep-PCR) fingerprinting and specifically detected in Ralstonia solanacearum race 4 strains (ginger, mioga, and curcuma isolates). One primer set (AKIF-AKIR) amplified a single band (165 bp) from genomic DNA obtained from all mioga and curcuma and some ginger isolates; another set (21F-21R) amplified one band (125 bp) from the other ginger isolates. These primer sets did not amplify the bands from genomic DNA of other R. solanacearum strains or of other related bacteria. PCR detection limit for the pathogen was 2 × 102 cfu.
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The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession numbers AB118756 and AB118757
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Horita, M., Yano, K. & Tsuchiya, K. PCR-based specific detection of Ralstonia solanacearum race 4 strains. J Gen Plant Pathol 70, 278–283 (2004). https://doi.org/10.1007/s10327-004-0126-7
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DOI: https://doi.org/10.1007/s10327-004-0126-7