Abstract
Fumaria agraria (Fumariaceae) is an annual plant used traditionally in Algeria for various medicinal purposes. Alkaloids extracts from areal parts were subjected to solvent fractionation and GCMS analysis. Rates of alkaloids recorded were respectively of 700 mg/100 g of Dry Weight of total alkaloids “TA”, 400 mg/100 g DWof neutral fraction “NF”, 40 mg/100 g DW of acid fraction “AF” and 250 mg/100 g DWof the basic fraction “BF”. Protopine was found to be the most potent alkaloid of the total extract (41.55%). Fractionation showed that Protopine was found essentially in the BF fraction (60.7%). Antiproliferative activities of total alkaloids extract and BF fraction were assessed on MCF-7 and MDAMB- 231 breast cancer and MCF10A mammary normal cell lines, by trypan blue exclusion essay. Both TA extract and BF fraction showed potent antiproliferative activities against MCF-7 breast cancer cells in varying degrees with respective IC50 of 47.8 ± 1.8 µg/ml and 17.6 ± 0.4 µM, without cell death induction but no impact was observed on normal MCF-10A cell growth. MCF7 Cell line was most sensitive (IC50 of 17,6 ± 0,4µM) to BF fraction effect than MDAMB-231 one (IC50 of 36,4 ± 0,6µM). Isoquinolein alkaloids of Fumaria agraria and specially the fraction which contain Protopine showed potent antiproliferative activity against breast cancer cells.
Résumé
Fumaria agraria (Fumariacae) est une plante annuelle utilisée traditionnellement en Algérie pour divers affections. Les extraits alcaloïdiques des parties aériennes de la plante ont été soumis à un fractionnement par solvant et à une analyse par GC-MS. Les taux d’alcaloïdes enregistrés ont été de 700 mg/100 g de poids sec (PS) pour les alcaloïdes totaux « TA », 400 mg/100 g PS pour la fraction neutre « NF », 40 mg/100 g PS pour la fraction acide « AF » et enfin de 250 mg/100 g PS en ce qui concerne la fraction basique « BF ». La Protopine s’est révélée comme étant l’alcaloïde majoritaire dans l’extrait total TA (41,55%) et comme le principal composant de la fraction BF (60,7%). L’évaluation de l’effet antiprolifératif de ATet FB par le test d’exclusion au bleu trypan, a été menée sur deux lignées cellulaires du cancer du sein MCF7 et MD-MB-231 et sur MCF10A, une lignée cellulaire non tumorale. TA et FB ont démontré tous les deux une activité antiproliférative variable vis-à-vis de MCF7 avec des IC50 respectives de 47,8 ± 1,8 µg/ml et 17,6 ± 0,4 µMsans mort cellulaire observée et sans impact sur la croissance de la lignée cellulaire normale MCF-10A. Par ailleurs la lignée MD-MB-231 s’est montrée moins sensible à l’action de la fraction FB avec une IC50 de 36,4 ± 0,6 µM. Les alcaloïdes isoquinoléïques de Fumaria agraria ont montré un effet antiprolifératif important vis-à-vis de lignées cellulaires de cancer du sein.
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Bougoffa-Sadaoui, K., Gontier, E., Telliez, M. et al. Characterization of isoquinolin alkaloids from Fumaria agraria and evaluation of their antiproliferative activity against human breast cancer cell lines. Phytothérapie 14, 188–195 (2016). https://doi.org/10.1007/s10298-015-0981-8
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DOI: https://doi.org/10.1007/s10298-015-0981-8