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The Zea mays glycine-rich RNA-binding protein MA16 is bound to a ribonucleotide(s) by a stable linkage

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Abstract

Expression of the gene encoding the maize glycine-rich RNA-binding protein MA16 is developmentally regulated and it is involved in environmental stress responses. The MA16 protein shows a wide spectrum of RNA-binding activities. On the basis of in vivo labelling, where a [32P]phosphate label was linked to the MA16 protein, Freire and Pages (Plant Mol Biol 29:797–807, 1995) suggested that the protein may be post-translationally modified by phosphorylation. However, further analysis showed that the [32P]phosphate label was sensitive to different treatments, suggesting that modification distinct from protein phosphorylation might occur in the MA16 protein. Biochemical analysis revealed that this [32P]phosphate labelling was resistant to phenol extraction and denaturing SDS-PAGE but sensitive to micrococcal nuclease, RNase A and RNase T1 treatments. The mobility of [35S] labelled MA16 protein on SDS-PAGE did not significantly changed after the nuclease treatments suggesting that the [32P]phosphate label associated to MA16 protein could be a ribonucleotide or a very short ribonucleotide chain. In addition, immunoprecipitation of labelled extracts showed that the ribonucleotide(s) linked to the MA16 protein was removed by phosphorolytic activity. This activity could be catalysed by a phosphate-dependent ribonuclease. The C-terminus of MA16 protein harbouring a glycine-rich domain was predicted to be an intrinsically disordered region.

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Abbreviations

MN:

Micrococcal nuclease

PNPase:

Polynucleotide phosphorylase

Pi:

Inorganic phosphate

PDX:

Phosphate-dependent exoribonuclease

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Acknowledgments

I wish to thank Dr. M. Pages for fruitful discussions, Ms. E. Vega for editing the manuscript, Dr. S. Burgess for English corrections and CSIC and CONICET for support.

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Authors and Affiliations

  1. Facultad de Ciencias Exactas, Físicas y Naturales, Instituto Multidisciplinario de Biología Vegetal, CONICET, Universidad Nacional de Córdoba, Edificio de Investigaciones Biológicas y Tecnológicas Av. Vélez Sarsfield 1611, 5000, Córdoba, Argentina

    Miguel Angel Freire

  2. Departamento de Genética Molecular de Plantas, Instituto de Biología Molecular de Barcelona, Jordi Girona-Salgado 18-26, 08034, Barcelona, Spain

    Miguel Angel Freire

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Correspondence to Miguel Angel Freire.

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Freire, M.A. The Zea mays glycine-rich RNA-binding protein MA16 is bound to a ribonucleotide(s) by a stable linkage. J Plant Res 125, 653–660 (2012). https://doi.org/10.1007/s10265-012-0476-8

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