Abstract
The study of AFLP analysis in Kandelia obovata, one of the major mangrove species in Japan, revealed the existence of a unique fragment showing stuttered peaks. We cloned this fragment and found a novel microsatellite locus. We report the method used for isolation and the polymorphic nature of this locus among the populations on Iriomote Island.
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References
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Giang LH, Hong PN, Tuan MS, Harada K (2003) Genetic variation of Avicennia marina (Forsk) Vierh (Avicenniaceae) in Vietnam revealed by microsatellite and AFLP markers. Genes Genet Syst 78:399–407
Habu Y, Fukada-Tanaka S, Hisatomi Y, Iida S (1997) Amplified restriction fragment length polymorphism-based mRNA fingerprinting using a single restriction enzyme that recognizes a 4-bp sequence. Biochem Biophys Res Commun 234:516–521
Hanski IA, Gilpin ME (1997) Metapopulation biology: ecology, genetics and evolution. Academic Press, Orlando
Macguire TL, Edwards KJ, Saenger P, Henry R (2000) Characterization and analysis of microsatellite loci in a mangrove species, Avicennia marina (Forsk) Vierh (Avicenniaceae). Theor Appl Genet 101:279–285
Macguire TL, Peakall R, Saenger P (2002) Comparative analysis of genetic diversity in the mangrove species Avicennia marina (Forsk) Vierh (Avicenniaceae) detected by AFLPs and SSRs. Theor Appl Genet 10:388–398
Sheue CR, Liu HY, Yong WH (2003) Kandelia obovata (Rhizophoraceae), a new species from Eastern Asia. Taxon 52:287–294
Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16
Acknowledgements
We thank Mr. Sosaku Ikeda for his technical assistance. This study was supported in part by Grants-in-Aid for Scientific Research (no. 13575001) from the Japan Society for Promotion of Science.
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Harada, K., Okaura, T., Giang, L.H. et al. A novel microsatellite locus isolated from an AFLP fragment in the mangrove species Kandelia obovata (Rhizophoraceae). J Plant Res 118, 49–51 (2005). https://doi.org/10.1007/s10265-004-0187-x
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DOI: https://doi.org/10.1007/s10265-004-0187-x