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Neurohistochemistry in molluscan species: focus on extracellular matrix

  • Original Paper
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Invertebrate Neuroscience

Abstract

Carbohydrate polymers with acid–base properties of extracellular matrix (ECM) have a critical impact on guidance, migration and synaptic transmission. The present study showed acid–base properties of ECM stained with histochemical techniques. The central nervous system (CNS) of mature terrestrial slug Semperula maculata and freshwater bivalve Lamellidens corrianus was selected to visualize sulphated and carboxylic strong acid groups. Protein and carbohydrate molecules were found scattered in three different regions, viz. periganglionic sheath, along with the basement lamina, interperikaryal space including the perineuronal net, and neuropil, in both the molluscan species. Sulphated proteoglycans were abundant in periganglionic sheath of S. maculata and also occurred in the neuropil of L. corrianus. The interperikaryal space contained carboxyl groups along with hyaluronic acid, and neuropil contained abundant glycogen moiety in both species. Comparing the SDS–PAGE of the neural tissue extracts revealed protein molecules that were found in the same fraction. The ECM of neuronal cells from S. maculata and L. corrianus presented different kinds of glycoproteins and proteoglycans, which showed its morphological as well as habitual differentiation.

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References

  • Benjamin PR, Staras K, Kemenes G (2000) A systems approach to the cellular analysis of associative learning in the pond snail Lymnaea. Learn. Mem 7:124–131

    Article  PubMed  CAS  Google Scholar 

  • Briickner G, Gogala M, Zei M, Biesold D (1983) Histochemical detection of anionic components in the cephalopods brain. Acta Histochem 74:91–102

    Article  Google Scholar 

  • Cassaro CMF, Dietrich CP (1977) Distribution of sulphated mucopolysaccharides in invertebrates. J Biol Chem 252:2254–2261

    PubMed  CAS  Google Scholar 

  • Chase R (2002) Behavior and its Neural Control in Gastropods Mollusks. Oxford University Press, Oxford

    Google Scholar 

  • Elekes K (1991) Serotonin-immunoreactive varicosities in the cell body region and neural sheath of the snail, Helix pomatia, ganglia: an electron microscopic immunocytochemical study. Neuroscience 42:583–591

    Article  PubMed  CAS  Google Scholar 

  • Elekes K, Nassel DR (1990) Distribution of FMRFamide-like immunoreactive neurons in the central nervous system of the snail, Helix pomatia. Cell Tissue Res 262:177–190

    Article  CAS  Google Scholar 

  • Harris HF (1900) On the rapid conversion of haematoxylin into haematein in staining reactions. J Appl Microsc 3:777

    Google Scholar 

  • Hartmann U, Maurer P (2001) Proteoglycans in the nervous system—the ques: for functional roles in vivo. Matrix Biol 20:23–35

    Article  PubMed  CAS  Google Scholar 

  • Hawkins RD, Kandel ER, Bailey CH (2006) Molecular mechanism of memory storage in Aplysia. Biol Bull 210:174–191

    Article  PubMed  CAS  Google Scholar 

  • Hernadi L, Elekes K, S-Rozsa K (1989) Serotonin-containing neurons in the central nervous system of the snail, Helix pomatia. Comparison of immunocytochemical and 5, 6-dihydroxytryptamine-labelling. Cell Tissue Res 257:313–323

    Article  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  • Lev R, Spicer SS (1964) Specific staining of sulphate groups with Alcian blue at pH J. Histochem Cytochem 12:309

    Article  CAS  Google Scholar 

  • Lopes-Lima M, Ribeiro I, Pinto RA, Machodo J (2005) Isolation, purification and characterization of glycosaminoglycans in the fluids of the mollusk Anodonta cygnea. Comp Biochem Phys A 141:319–326

    Article  CAS  Google Scholar 

  • Machado J, Castilho F, Coimbra J, Sa C, Monterio E (1988) Ultrastructural and cytochemical studies in the mantle of Anodonta cygnea. Tissue Cell 20:797–807

    Article  PubMed  CAS  Google Scholar 

  • Mc Manus JFA (1946) Histological demonstration of acid mucin after periodic acid. Nature 158:202

    Article  CAS  Google Scholar 

  • Medeiros GF, Mendes A, Castro RAB, Bad EC, Nader HB, Dietrich CP (2000) Distribution of sulphated glycoaminoglycans in the animal kingdom: widespread occurrence of heparin—like compounds in invertebrates. Biochim Biophys Acta 1475:287–294

    Article  PubMed  CAS  Google Scholar 

  • Mowry R, Winkler CH (1956) The coloration of acidic carbohydrates of bacteria and fungi in tissue sections with special reference to capsules of Cryptococcus neoformans, Pneumococcus and Staphylococcus. Am J Pathol 36:628–629

    Google Scholar 

  • Poncet JM, Serpentini A, Thiebot B, Villers C, Bocquet J, Boucaud-Camou E, Lebet JM (2000) In vitro synthesis of proteoglycans and collagen in primary cultures of mantle cells from the nacreous mollusk, Halilots tuberculata; a new modle for study of molluscan extracellular matrix. Mar Biotechnol 2:387–398

    PubMed  CAS  Google Scholar 

  • Reichardt LF, Tomaselli KJ (1991) Extracellular matrix molecules and their functions in neuronal developments. Annu Rev Neurosci 14:531–570

    Article  PubMed  CAS  Google Scholar 

  • Rutka JT, Apodaca G, Stern R, Rosenblum M (1999) The Extracellular matrix of the central and peripheral nervous system: Structure and function. J Neurosurg 69:155–170

    Google Scholar 

  • Serfőző Z, Elekes K (2007) Histochemistry of the Extracellular matrix in the snail nervous system. Clin Neurosci 60:173–176

    Google Scholar 

  • Serfőző Z, Elekes K (2009) Lectin-binding glycoproteins in the developing and adult snail CNS, Brian. Struct Funct 214:67–78

    Article  Google Scholar 

  • Serfőző Z, Elekes K (2010) Chemical properties of the extracellular matrix of the snail nervous system: a comprehensive study using a combination of histochemical techniques. Micron 41:467–471

    Google Scholar 

  • Spicer SS (1960) A correlative study of the histochemical properties of rodent acid muco-polysaccharides. J Histochem Cytochem 8:18–34

    Article  PubMed  CAS  Google Scholar 

  • Sykova E (2004) Extrasynaptic volume transmission and diffusion parameters of the extracellular space. Neuroscience 129:861–876

    Article  PubMed  CAS  Google Scholar 

  • Volpi N, Maccari F (2003) Purification and characterization of hyaluronic acid from the mollusc bivalve Mytilus galloprovincialis. Biochemie 85:619–625

    Article  CAS  Google Scholar 

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Acknowledgments

Authors are thankful to University Grant Commission, New Delhi, for providing financial support under Major Research Project. Authors are also thankful to the Head, Department of Zoology, Shivaji University, Kolhapur, for providing facilities in the progress of work.

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Authors and Affiliations

  1. Department of Zoology, Shivaji University, Kolhapur, 416 004, India

    Nitin A. Kamble & Sunil R. Londhe

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  1. Nitin A. Kamble
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  2. Sunil R. Londhe
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Correspondence to Nitin A. Kamble.

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Kamble, N.A., Londhe, S.R. Neurohistochemistry in molluscan species: focus on extracellular matrix. Invert Neurosci 12, 129–138 (2012). https://doi.org/10.1007/s10158-012-0139-4

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