Abstract
Background
This study was done to investigate the DNA binding ability of a diagnostic biomarker, 45-kDa calcium oxalate monohydrate (COM) binding protein, isolated from human kidney and its effect on transcription.
Methods
The 45-kDa COM binding protein was isolated and purified from human kidney. The subcellular localization of the protein and the amino acid composition of the protein were analyzed. Oxalate-binding activity in the presence or absence of DNA was determined. The possibility of forming DNA-protein adducts was checked by diethylaminoethyl (DEAE)-Sephadex column chromatography. The effect of the protein on in vitro transcription was also studied.
Results
The isolated 45-kDa protein was found to be basic in nature and its AACompIdent analysis showed it to be related to known transcription factors. The protein was found to be present in kidney cytosol and nucleus. The decreased oxalate binding activity in the presence of the DNA, and the shift in the DEAE-Sephadex elution profile established the DNA-binding ability of the protein. The in vitro transcription assay demonstrated the repression effect of the protein on gene expression during hyperoxaluria.
Conclusions
Transcriptional repression by the 45-kDa COM binding protein in an in vitro transcription assay system was reduced in the presence of oxalate. Hence, altered expression of certain genes involved in the prognosis of urolithiasis might be mediated by this 45-kDa protein.
Similar content being viewed by others
References
DJ Kok SR Khan (1994) ArticleTitleCalcium oxalate nephrolithiasis, a free or fixed particle disease Kidney Int 46 847–54 Occurrence Handle1:STN:280:DyaK2M%2FovFejsQ%3D%3D Occurrence Handle10.1038/ki.1994.341 Occurrence Handle7996806
R Selvam VP Lakshmi (1996) ArticleTitleOccurrence of histone-related oxalate binding in rat liver nucleus Mol Cell Biochem 156 93–100 Occurrence Handle1:CAS:528:DyaK28XisFaku7g%3D Occurrence Handle10.1007/BF00426330 Occurrence Handle9095464
JS Grewal JY Tsai SR Khan (2005) ArticleTitleOxalate inducible AMBP gene and its regulatory mechanism in renal tubular epithelial cells Biochem J 387 609–16 Occurrence Handle1:CAS:528:DC%2BD2MXjsFyrtbk%3D Occurrence Handle10.1042/BJ20041465 Occurrence Handle15533056 Occurrence Handle1134990
E Konya T Umekawa M Iguchi T Kurita (2003) ArticleTitleThe role of osteopontin on calcium oxalate crystal formation Eur Urol 43 564–71 Occurrence Handle1:CAS:528:DC%2BD3sXmt1Wntbs%3D Occurrence Handle10.1016/S0302-2838(03)00088-5 Occurrence Handle12706004
R Selvam K Kannabiran (1996) ArticleTitleCharacterisation of nuclear oxalate binding of rat and human kidney J Urol 156 237–42 Occurrence Handle1:CAS:528:DyaK28Xktlyhu7s%3D Occurrence Handle10.1016/S0022-5347(01)66008-7 Occurrence Handle8648814
R Selvam S Devaraj (1997) ArticleTitleOxalate binding protein of rat and human mitochondria: studies on its properties Indian J Biochem Biophys 34 470–8 Occurrence Handle1:CAS:528:DyaK1cXks1elsQ%3D%3D Occurrence Handle9594427
P Sivakamasundari R Sakthivel P Kalaiselvi R Selvam P Varalakshmi (2004) ArticleTitleExpression of nuclear pore complex oxalate binding protein p62 in experimental hyperoxaluria Nephron Exp Nephrol 97 e106–e14 Occurrence Handle1:CAS:528:DC%2BD2cXmsV2it7w%3D Occurrence Handle10.1159/000078644 Occurrence Handle15292681
D Asokan P Kalaiselvi P Varalakshmi (2004) ArticleTitleModulatory effect of the 23 kDa calcium oxalate monohydrate binding protein on calcium oxalate stone formation during oxalate stress Nephron Physiol 97 23–30 Occurrence Handle10.1159/000077599
P Kalaiselvi R Selvam (2001) ArticleTitleEffect of experimental hyperoxaluria on renal calcium oxalate monohydrate binding proteins in the rat Br J Urol 87 110–6 Occurrence Handle1:CAS:528:DC%2BD3MXht1Wjs74%3D Occurrence Handle10.1046/j.1464-410x.2001.00972.x
D Asokan P Kalaiselvi SM Farooq P Varalakshmi (2004) ArticleTitleCalcium oxalate monohydrate binding protein: a diagnostic biomarker for calcium oxalate kidney stone formers Urol Res 32 357–61 Occurrence Handle1:CAS:528:DC%2BD2cXovVCmsro%3D Occurrence Handle10.1007/s00240-004-0430-x Occurrence Handle15365653
S Laxmanan R Selvam CJ Mahle M Menon (1986) ArticleTitleBinding of oxalate to mitochondrial inner membranes of rat and human kidney J Urol 135 862–5 Occurrence Handle1:CAS:528:DyaL28XitVCqt7w%3D Occurrence Handle10.1016/S0022-5347(17)45877-0 Occurrence Handle3959219
GP Talwar (1983) Non-isotopic immunoassays and their applications Vikas Publishing House Private New Delhi 55–62
GI Bell JH Karam WJ Rutter (1981) ArticleTitlePolymorphic DNA region adjacent to the 5′ end of the human insulin gene Proc Natl Acad Sci USA 78 5759–63 Occurrence Handle1:CAS:528:DyaL3MXlvVClt7w%3D Occurrence Handle10.1073/pnas.78.9.5759 Occurrence Handle6272317 Occurrence Handle348853
W Marzlutt R Huang (1984) RNA transcription in isolated nuclei BH Hammes SJ Higgins (Eds) Transcription and translation – a practical approach IRL Washington 141–59
G Blobel VR Porter (1966) ArticleTitleNuclei from rat liver: isolation method that combines purity with high yield Science 154 1662–5 Occurrence Handle1:CAS:528:DyaF2sXot1Ghsw%3D%3D Occurrence Handle10.1126/science.154.3757.1662 Occurrence Handle5924199
K Burton (1956) ArticleTitleA study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid Biochem J 62 315–23 Occurrence Handle1:CAS:528:DyaG28XktVyktQ%3D%3D Occurrence Handle10.1042/bj0620315 Occurrence Handle13293190 Occurrence Handle1215910
B Baggio G Gambaro F Marchini E Cicerrello A Borstti (1984) ArticleTitleRaised transmembrane oxalate flux in red blood cells in idiopathic calcium oxalate nephrolithiasis Lancet 2 12–3 Occurrence Handle1:STN:280:DyaL2c3ksVOltQ%3D%3D Occurrence Handle10.1016/S0140-6736(84)91998-6 Occurrence Handle6145933
HK Koul SK Thind R Nath (1991) ArticleTitleOxalate binding of rat intestinal brush border membrane in pyridoxine deficiency: a kinetic study Biochim Biophys Acta 1064 184–8 Occurrence Handle1:CAS:528:DyaK3MXktVOns7k%3D Occurrence Handle10.1016/0005-2736(91)90300-W Occurrence Handle2036434
R Selvam P Kalaiselvi (2000) ArticleTitleA novel basic protein from human kidney which inhibits calcium oxalate crystal growth Br J Urol 86 7–13 Occurrence Handle1:CAS:528:DC%2BD3cXlsV2lsb0%3D Occurrence Handle10.1046/j.1464-410x.2000.00725.x
L Shapiro PE Scherer (1998) ArticleTitleThe crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor Curr Biol 8 335–8 Occurrence Handle1:CAS:528:DyaK1cXhvFaru7w%3D Occurrence Handle10.1016/S0960-9822(98)70133-2 Occurrence Handle9512423
C Elam L Hesson MD Vos K Eckfeld CA Ellis A Bell et al. (2005) ArticleTitleRRP22 is a farnesylated, nucleolar, Ras-related protein with tumor suppressor potential Cancer Res 65 3117–25 Occurrence Handle1:CAS:528:DC%2BD2MXjt1yktr0%3D Occurrence Handle10.1158/0008-5472.CAN-04-0749 Occurrence Handle15833841
LM Eisenberg CA Eisenberg (2006) ArticleTitleWnt signal transduction and the formation of the myocardium Dev Biol 293 305–15 Occurrence Handle1:CAS:528:DC%2BD28XksVOnu7o%3D Occurrence Handle10.1016/j.ydbio.2006.02.014 Occurrence Handle16563368
R Selvam P Kalaiselvi (2003) ArticleTitleOxalate binding proteins in calcium oxalate nephrolithiasis Urol Res 31 242–56 Occurrence Handle1:CAS:528:DC%2BD3sXmsFykt7s%3D Occurrence Handle10.1007/s00240-003-0316-3 Occurrence Handle12856168
RM Mannermaa J Oikarinen (1992) ArticleTitleNucleoside triphosphate binding and hydrolysis by histone H1 Biochem Biophys Res Commun 182 309–17 Occurrence Handle1:CAS:528:DyaK38XlsVSjtA%3D%3D Occurrence Handle10.1016/S0006-291X(05)80146-9 Occurrence Handle1731790
PJ Laybourn JT Kadonaga (1991) ArticleTitleRole of nucleosomal cores and histone H1 regulation of transcription by RNA polymerase III Science 254 238–45 Occurrence Handle1:CAS:528:DyaK3MXmsV2gtb4%3D Occurrence Handle10.1126/science.1718039 Occurrence Handle1718039
M Ivanchenko J Zlatonova K Von holde (1997) ArticleTitleHistone H1 preferentially binds to superhelical DNA molecule of higher compaction Biophys J 72 1388–95 Occurrence Handle1:CAS:528:DyaK2sXhsVeitL0%3D Occurrence Handle10.1016/S0006-3495(97)78785-X Occurrence Handle9138584 Occurrence Handle1184521
M Grunstein (1990) ArticleTitleNucleosomes – regulators of transcription Trends Genet 6 395–400 Occurrence Handle1:CAS:528:DyaK3MXhtFGrtr8%3D Occurrence Handle10.1016/0168-9525(90)90299-L Occurrence Handle2087781
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Kandaswamy Veena, C., Asokan, D., Kalaiselvi, P. et al. Transcriptional repression mediated by 45-kDa calcium oxalate monohydrate binding protein. Clin Exp Nephrol 11, 196–201 (2007). https://doi.org/10.1007/s10157-007-0477-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10157-007-0477-4