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Genetic mutation analysis of the malignant transformation of sinonasal inverted papilloma by targeted amplicon sequencing

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Abstract

Background

The mechanism underlying the malignant transformation of inverted papilloma (IP) has not yet been elucidated.

Methods

To clarify the genes responsible for the malignant transformation, we analyzed 10 cases of IP, 8 of IP with dysplasia, and 11 of squamous cell carcinoma (SCC) by targeted amplicon sequencing.

Results

The number of mutant genes increased in the order of IP < dysplasia < SCC. Significant differences were observed in the mutation rates of three genes (KRAS, APC and STK11) in particular. TP53 was altered frequently in each group and might be involved in malignant transformation based on to the site of the mutation. A comparison of the genetic variants by region of IP tissue among patients with IP alone, and those with dysplasia or SCC revealed significant differences in the mutation rate of the KRAS gene.

Conclusion

Identification of genetic mutations in KRAS is effective for predicting the malignant transformation of IP.

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Authors and Affiliations

  1. Department of Otolaryngology, Head and Neck Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Kita 15, Nishi 7, Kita-ku, Sapporo, 060-8638, Japan

    Shinichiro Yasukawa, Satoshi Kano, Yuji Nakamaru, Dai Takagi, Takatsugu Mizumachi, Masanobu Suzuki, Takayoshi Suzuki, Akira Nakazono & Akihiro Homma

  2. Department of Otolaryngology, Yokohama City University Medical Center, Yokohama, Japan

    Hiromitsu Hatakeyama

  3. Department of Cancer Pathology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan

    Shinya Tanaka

  4. Department of Cancer Center, Keio University Hospital, Tokyo, Japan

    Hiroshi Nishihara

Authors
  1. Shinichiro Yasukawa
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  2. Satoshi Kano
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  3. Hiromitsu Hatakeyama
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  4. Yuji Nakamaru
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  5. Dai Takagi
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  6. Takatsugu Mizumachi
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  7. Masanobu Suzuki
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  8. Takayoshi Suzuki
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  9. Akira Nakazono
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  10. Shinya Tanaka
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  11. Hiroshi Nishihara
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  12. Akihiro Homma
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Corresponding author

Correspondence to Satoshi Kano.

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Yasukawa, S., Kano, S., Hatakeyama, H. et al. Genetic mutation analysis of the malignant transformation of sinonasal inverted papilloma by targeted amplicon sequencing. Int J Clin Oncol 23, 835–843 (2018). https://doi.org/10.1007/s10147-018-1296-1

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  • DOI: https://doi.org/10.1007/s10147-018-1296-1

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