Abstract
Wheat (Triticum aestivum) is one of the most important food crops worldwide, providing up to 20% of the caloric intake per day. Developing high-yielding wheat cultivars with tolerance against abiotic and biotic stresses is important to keep up with the increasing human population. Tiller number is one of the major yield-related traits, directly affecting the number of grains produced per plant; however, only a small number of QTL and underlining genes have been identified for this important factor. Identification of novel genetic variation underlying contrasting traits and their precise genetic mapping in wheat is considered difficult due to the complexity and size of the genome; however, advancements in genomic resources have made efficient gene localization more possible. In this study, we report the characterization of a novel tillering number gene using a mutant identified in the forward genetic screen of an ethyl methane sulfonate (EMS)-treated population of cv. “Jagger.” By crossing the low tillering mutant with the Jagger wild-type plant, we generated an F2 population and used the MutMap approach to identify a novel physical interval on 11 Mb on chromosome 2DS. Using an F2 population of 442 gametes and polymorphic SNP markers, we were able to delineate the tin6 locus to a 2.1 Mb region containing 22 candidate genes.
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Data availability
DNA raw sequencing reads are available under the NCBI BioProject ID: PRJNA954413.
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Funding
Authors thankfully acknowledge financial support from the United States Department of Agriculture—National Institute of Food and Agriculture (award # 2020–67013-31460).
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VT and JP conceived the idea of the project and designed the experiments. AS and SW performed experiments. AS and ISY performed data analysis. JP and NR provided resources for experiments. AS, VT, and ISY wrote the manuscript with inputs from the co-authors. All co-authors read and approved the manuscript.
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Key message
This work reports an integrated forward genetic approach that combines whole genome sequencing of mutant bulk to identify a novel tiller number gene in hexaploid wheat.
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Supplementary file1 Supplementary Figure 1: Linear plot showing average SNP indexes across all chromosomes. (PNG 690 kb)
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Supplementary file2 Supplementary Figure 2: Example of 10 recombinants and Jagger and tin6 control using the tin6_18.3 marker. (PNG 519 kb)
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Supplementary file4 Supplementary Figure 4: Circos plot showing synteny between Jagger (blue) and Chinese Spring (green). Blue lines show Jagger homologs on Chinese Spring whereas red lines show where one chromosome of Jagger is having match on another nonhomologous chromosome of Chinese Spring. (PNG 3246 kb)
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Schoen, A., Yadav, I., Wu, S. et al. Identification and high-resolution mapping of a novel tiller number gene (tin6) by combining forward genetics screen and MutMap approach in bread wheat. Funct Integr Genomics 23, 157 (2023). https://doi.org/10.1007/s10142-023-01084-2
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DOI: https://doi.org/10.1007/s10142-023-01084-2