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Expression Profiling Analysis of the microRNA Response of Cynoglossus semilaevis to Vibrio anguillarum and Other Stimuli

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Abstract

To investigate the roles of microRNAs (miRNA) of Cynoglossus semilaevis in response to Vibrio anguillarum that were previously identified using high-throughput sequencing, microarray analyses was performed on three small RNA libraries (CG, NOSG, and HOSG) prepared from C. semilaevis immune tissues. In total, of 1279 designed probes, 739 (57.78 %) were detectable. The expression levels of these miRNAs were analyzed using pairwise comparisons among the three libraries, and a total of 99 miRNAs were observed to be significantly differentially expressed. The expression patterns of 10 differentially expressed miRNAs were validated by real-time quantitative PCR (RT-qPCR). In addition, expression of miR-142-5p, miR-223, and miR-181a in response to V. anguillarum at numerous time-points in four tissues, as well as the responses to lipopolysaccharide (LPS), polyinosinic:polycytidylic acid (poly I:C), peptidoglycan (PGN), and red-spotted grouper nervous necrosis virus (RGNNV) in head kidney cells, were studied by qRT-PCR. Taken together, all of the expression profiles showed significant differences compared to the control group; both similarities and differences in the expression responses to the same pathogen were observed. Collectively, these findings highlighted the putative roles for miRNAs in the context of the innate immune response of C. semilaevis exposing to pathogens and that further studies are needed to understand the molecular mechanisms of miRNA regulation in C. semilaevis host–pathogen interactions.

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Authors and Affiliations

  1. Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, People’s Republic of China

    Guangye Gong, Zhenxia Sha, Songlin Chen, Hui Yan, Yadong Chen & Tianzi Wang

  2. Marine Science and Engineering College, Qingdao Agriculture University, Qingdao, 266109, People’s Republic of China

    Chao Li

  3. College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, People’s Republic of China

    Hui Yan

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  1. Guangye Gong
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  2. Zhenxia Sha
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  3. Songlin Chen
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  4. Chao Li
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  5. Hui Yan
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  6. Yadong Chen
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  7. Tianzi Wang
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Correspondence to Zhenxia Sha or Songlin Chen.

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Gong, G., Sha, Z., Chen, S. et al. Expression Profiling Analysis of the microRNA Response of Cynoglossus semilaevis to Vibrio anguillarum and Other Stimuli. Mar Biotechnol 17, 338–352 (2015). https://doi.org/10.1007/s10126-015-9623-2

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  • DOI: https://doi.org/10.1007/s10126-015-9623-2

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