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Antiviral Specificity of the Solea senegalensis Mx Protein Constitutively Expressed in CHSE-214 Cells

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Abstract

Interferons play a key role in fish resistance to viral infections by inducing the expression of antiviral proteins, such as Mx. The aim of the present study was to test the antiviral activity of the Senegalese sole Mx protein (SsMx) against RNA and DNA viruses pathogenic to fish, i.e. the infectious pancreatic necrosis virus (IPNV, dsRNA), the viral haemorrhagic septicaemia virus (VHSV, ssRNA), and the European sheatfish virus (ESV, dsDNA), using a CHSE-214 cell clone expressing this antiviral protein. A strong inhibition of IPNV and VHSV replication was recorded in SsMx-expressing cells, as has been shown by the virus yield reduction and the decrease in the synthesis of the viral RNA encoding the polyprotein (for IPNV) and the nucleoprotein (for VHSV). The titres of these viruses replicating on SsMx-expressing cells were 100 times lower than those recorded on non-transfected cells. In contrast, SsMx did not inhibit ESV replication since no significant differences were observed regarding the virus yield or the major capsid protein gene transcription in transfected and non-transfected cells.

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Acknowledgments

The authors want to thank CP Dopazo and K Way for supplying some of the viruses used in this work. This study has been funded by the projects P09-CVI-4579, from Junta de Andalucía (Proyectos de Excelencia de la Junta de Andalucía), and BioAnd 08/7/L2.5. D Alvarez-Torres was supported by a fellowship from Junta de Andalucía (Proyecto de Excelencia P09-CVI-4579).

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Correspondence to M. Carmen Alonso.

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Alvarez-Torres, D., Garcia-Rosado, E., Fernandez-Trujillo, M.A. et al. Antiviral Specificity of the Solea senegalensis Mx Protein Constitutively Expressed in CHSE-214 Cells. Mar Biotechnol 15, 125–132 (2013). https://doi.org/10.1007/s10126-012-9478-8

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  • DOI: https://doi.org/10.1007/s10126-012-9478-8

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