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Hypersalinity and Hydrogen Peroxide Upregulation of Gene Expression of Antioxidant Enzymes in Ulva fasciata Against Oxidative Stress

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Abstract

The modulation of manganese superoxide dismutase (MnSOD), FeSOD, ascorbate peroxidase (APX), glutathione reductase (GR), and catalase (CAT) gene expression and activities and antioxidants in Ulva fasciata against hypersalinity (90‰)-induced oxidative stress was studied. Increases in H2O2 contents but no changes in lipid peroxidation and protein carbonyl group contents suggest oxidative damage did not occur in 90‰ condition. Antioxidants were consumed for reactive oxygen species (ROS) scavenging indicated by decreased ascorbate and glutathione contents by 90‰. Antioxidant enzymes were differently expressed by 90‰ for ROS removal. MnSOD activity and transcript increased 1 h after 90‰ treatment with a peak at hour 3, while FeSOD activity increased fast to the plateau after 1 h and its transcript increased after 3 h. APX activity increased 1 h after 90‰ but its transcript rose till 3 h, and GR activity increased after 1 h with a peak at hour 3 but its transcript increased till 3 h. CAT activity and transcript increased after 12 h. Enzyme activity is transcriptionally regulated by 90‰ except a fast increase in FeSOD, APX, and GR activities during 1 h. APX is responsible for early H2O2 decomposition while CAT scavenges H2O2 in the later period. The inhibition of 90‰ induced increase of H2O2 content and FeSOD activity and transcript by treatment of a H2O2 scavenger, dimethylthiourea, and the increase of FeSOD transcript of 30‰ grown thalli by H2O2 treatment suggest that H2O2 mediates the upregulation of FeSOD by hypersalinity while other enzymes is modulated by factors other than H2O2.

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Acknowledgments

The grant was from the National Science Council (NSC 96-2311-B-110-001), Executive Yuan, Taiwan, Republic of China. We thank Prof. CL Tsai in the Department of Marine Biotechnology and Resource, National Sun Yat-sen University, Kaohsiung, Taiwan, Republic of China for kindly support of ABI PRISM 7000 Sequence Detection System.

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Authors and Affiliations

  1. Institute of Marine Biology, National Sun Yat-sen University, Kaohsiung, Taiwan, 80424, Republic of China

    Ming-Shiuan Sung, Yi-Ting Hsu, Yuan-Ting Hsu, Tzure-Meng Wu & Tse-Min Lee

  2. The Kuroshio Research Group of the Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung, Taiwan, 80424, Republic of China

    Ming-Shiuan Sung, Yi-Ting Hsu, Yuan-Ting Hsu, Tzure-Meng Wu & Tse-Min Lee

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  1. Ming-Shiuan Sung
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  2. Yi-Ting Hsu
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Correspondence to Tse-Min Lee.

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Sung, MS., Hsu, YT., Hsu, YT. et al. Hypersalinity and Hydrogen Peroxide Upregulation of Gene Expression of Antioxidant Enzymes in Ulva fasciata Against Oxidative Stress. Mar Biotechnol 11, 199–209 (2009). https://doi.org/10.1007/s10126-008-9134-5

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  • DOI: https://doi.org/10.1007/s10126-008-9134-5

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